针刺加服乳乐冲剂对乳腺增生大鼠血清催乳素、雌二醇、孕酮及其受体在乳腺组织中表达的影响  

Effect of Acupuncture Combined with Gavage of Rule Granule on Serum Prolactin,Estradiol and Progestone Contents and Their Receptor Expression in Breast in Mammary Gland Hyperplasia Rats

作　　者：赵娴[1] 乔迪 侯咪 ZHAO Xian;QIAO Di;HOU Mi(Department of Science and Engineering,Shaanxi University of Traditional Chinese Medicine,Xianyang 712046,Shaanxi Province,China;Department of Rehabilitation,Lin fen City 4^th People＇s Hospital,Lin fen 041000,Shanxi Province;Department of Rehabilitation,Yanan City Hospital of Traditional Chinese Medicine,Yan＇an 716000,Shaanxi Province)

机构地区：[1]陕西中医药大学学工部,陕西咸阳712046 [2]临汾市第四人民医院康复科,山西临汾041000 [3]延安市中医医院康复科,陕西延安716000

出　　处：《针刺研究》2018年第10期645-650,共6页Acupuncture Research

基　　金：国家自然科学基金面上项目(No.81473782);陕西省中医药管理局项目(No.13-LC 068)

摘　　要：目的:观察针刺结合乳乐冲剂对乳腺增生(MGH)大鼠血清催乳素(PRL)、雌二醇(E2)、孕酮(P)及其受体PRLR、ER、PR在乳腺组织中表达的影响,探讨其部分作用机制,为筛选更有效的临床治疗方案提供实验依据。方法:将雌性SD大鼠随机分为空白组10只、模型组10只、针刺组11只、乳乐组10只和针药组9只。采用外源性激素联合刺激制备MGH模型。针刺组取甲、乙两组穴,1组/次,同时予以1.5mL/100g蒸馏水灌胃干预;乳乐组灌服乳乐冲剂,1.5mL/100g,同时予以抓取干预;针药组治疗方法同针刺组和乳乐组。治疗及干预措施均为1次/d。连续治疗30d后腹主动脉采血,ELISA法检测血清PRL、E2、P水平;光镜下观察乳腺组织HE染色切片和SABC免疫组化法检测乳腺组织PRLR、ER、PR阳性表达情况。结果:与空白组比较,模型组大鼠乳腺组织增生明显,血清PRL、E2含量及乳腺组织PRLR、ER、PR阳性表达积分显著升高(P<0.01),P含量显著降低(P<0.01);与模型组比较,各治疗组大鼠乳腺组织增生均有改善,PRL、E2含量及PRLR、ER、PR阳性表达积分均显著降低(P<0.05,P<0.01),P含量显著升高(P<0.05,P<0.01);且针药组PRL、E2含量及PRLR、ER、PR阳性表达积分较针刺组、乳乐组降低更显著(P<0.05),P含量的升高也更显著(P<0.05)。结论:针刺、乳乐冲剂、针药结合的治疗方法,对外源性雌、孕激素所致大鼠MGH均有效,其机制可能与降低血清PRL、E2含量,升高P含量,抑制PRL与PRLR的结合,降低乳腺组织ER、PR表达水平,从而间接抑制E2水平有关。针药组总体疗效更为显著。Objective To observe the effect of acupuncture combined with medication（Rule Granule）on serum prolactin（PRL）,estradiol（E2）and progestone（P）contents and expressions of prolactin receptor（PRLR）,estrogen receptor（ER）and progestrone receptor（PR）proteins in mammary gland（MG）tissues of MG hyperplasia（MGH）rats,so as to explore its mechanisms underlying improvement of MGH.Methods A total of 55 female SD rats were randomized into blank control group（n=10）,model group（n=10）,acupuncture group（n=11）,medication group（n=10）,and acupuncture plus medication group（n=9）.The MGH model was established by muscular injection of E2 benzoate（0.5 mg/kg,once daily for 20 days）,followed by injection of P（5 mg/kg）into the lateral muscle of the hind-limb,once daily for 5 days,and the rats of the control group were treated by muscular injection of normal saline at the same site and in the same procedures.Acupoint group A composed of bilateral Tianzhong（SI 11）,Ganshu（BL 18）and Zusanli（ST 36）,and group B composed of Tanzhong（CV 17）,and bilateral Wuyi（ST 15）and Hegu（LI 4）were alternatively punctured with filiform needles and stimulated swiftly by twirling the needle in each acupoint for 20-30 sec,once daily for 30 days.Rats of the medication group and acupuncture ＋medication group were treated by gavage of Rule Granule fluid containing Baishao（Paeonia Iactiflora Pall）,Danggui（Angelica sinensis）,etc.（1.5 mL/100 g for each rat）and those of the other groups treated by gavage of distilled water（1.5 mL/100 g）.The treatment was given once daily for continuous 30 d.At the end of the experiments,the rats＇ abdominal aorta blood was collected for assaying the contents of serum PRL,E2 and P with ELISA,and the pathological changes of breast tissue（the left 2 nd pairs）were observed under microscope after sectioning and H.E.staining.The expression of PRLR,ER and PR proteins in the breast tissue was detected by immunohistochemistry.Results After mode

关 键 词：乳腺增生病 针药结合 乳腺组织形态 性激素含量 性激素受体表达 

分 类 号：R245.31[医药卫生—针灸推拿学]