2015年版《中华人民共和国药典》收载的鹿源药材中硫酸软骨素含量分析  

Analysis of chondroitin sulfate content in deer related medicines in Pharmacopoeia of the People's Republic of China 2015

作　　者：宫瑞泽 王燕华[1,2] 陈宝[1,2] 孙印石[1] GONG Rui-ze;WANG Yan-hua;CHEN Bao;SUN Yin-shi(Institute of Special Wild Economic Animal and Plant Science,Chinese Academy of Agricultural Sciences,Changchun 130112,China;Chinese Medicinal Materials College,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]中国农业科学院特产研究所,长春130112 [2]吉林农业大学中药材学院,长春130118

出　　处：《药物分析杂志》2018年第10期1711-1718,共8页Chinese Journal of Pharmaceutical Analysis

基　　金：吉林省科技发展计划项目(20170309002YY;20170311027YY;20180201076YY);中国农业科学院科技创新工程项目(CAAS-ASTIP-2016-ISAPS)

摘　　要：目的:研究2015年版《中华人民共和国药典》(简称《中国药典》)收载的鹿源药材中硫酸软骨素的含量差异。方法:采用稀碱-浓盐法提取,硫酸软骨素ABC酶酶解样品中的硫酸软骨素,应用高效液相色谱法对样品中的总硫酸软骨素及硫酸软骨素A、B、C含量进行测定;色谱条件:Hypersil SAX强阴离子硅胶色谱柱(4.6 mm×250 mm,5μm),以2 mol·L^(-1)的氯化钠溶液(盐酸调pH至3.5)和水(盐酸调pH至3.5)为流动相,梯度洗脱,流速1.0 mL·min^(-1),检测波长232 nm,柱温40℃。结果:梅花鹿鹿茸、鹿角(鹿角脱盘)、鹿角胶(鹿角脱盘胶)、鹿角霜(鹿角脱盘霜)中硫酸软骨素含量分别为2.06、0.46(0.42)、0.93(1.10)、0.22(0.17)g·kg^(-1);马鹿鹿茸、鹿角(鹿角脱盘)、鹿角胶(鹿角脱盘胶)、鹿角霜(鹿角脱盘霜)中硫酸软骨素含量分别为1.83、0.41(0.34)、0.91(0.87)、0.19(0.15)g·kg^(-1)。结论:《中国药典》收载的鹿源药材中硫酸软骨素的含量差异很大,鹿茸中的最高,鹿角胶、鹿角、鹿角霜中的依次递减。Objective：To study the differences of chondroitin sulfate contents in deer related medicines in Pharmacopoeia of the People＇s Republic of China（Chinese Pharmacopoeia） 2015.Methods：Chondroitin sulfates from samples was extracted by dilute alkali-concentrated salt method.Then, chondroitin sulfate was digested by chondroitinase ABC.The contents of total chondroitin sulfate and chondroitin sulfate A, B and C in the samples were determined by HPLC.The separation was performed on a Hypersil SAX strong anionic silica column （4.6 mm×250 mm, 5 μm） with 2 mol·L^-1 sodium chloride solution （pH adjusted to 3.5 with hydrochloric acid） and water （pH adjusted to 3.5 with hydrochloric acid） as the mobile phase at a flow rate of 1.0 mL·min-1.The detection wavelength was 232 nm and the column temperature was set at 40℃.Results：The contents of chondroitin sulfate in Cervi Cornu Pantotrichum, Cervi Cornu（antlers plate）, Cervi Cornu Colla （antlers plate colla）, Cervi Cornu and Degelatinatum（antlers plate degelatinatum） of Cervus nippon Temminck were 2.06, 0.46（0.42）, 0.93（1.10） and 0.22（0.17） g·kg^-1, respectively.The contents of chondroitin sulfate in Cervi Cornu Pantotrichum, Cervi Cornu（antlers plate）, Cervi Cornu Colla （antlers plate colla）, and Cervi Cornu Degelatinatum（antlers plate degelatinatum） of Cervus elaphus Linnaeus were 1.83, 0.41（0.34）, 0.91（0.87） and 0.19（0.15） g·kg^-1, respectively.Conclusion：There are significant differences in the contents of chondroitin sulfate in deer related medicines in Chinese Pharmacopoeia, the contents of chondroitin sulfate in Cervi Cornu Pantotrichum were the highest, the contents of chondroitin sulfate in Cervi Cornu Colla, Cervi Cornu and Cervi Cornu Degelatinatum were descending in turn.

关 键 词：鹿源药材 梅花鹿 马鹿 鹿茸 鹿角 鹿角胶 鹿角霜 鹿角脱盘 鹿角脱盘胶 鹿角脱盘霜 酸性粘多糖 硫酸软骨素 含量分析 稀碱-浓盐提取 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]