高效液相色谱法同时测定蒙药复方述达格-4中6个成分的含量  

Simultaneous determination of six components in Mongolian medicine compound formula Shudage-4 by HPLC

作　　者：信莎莎 杜银飞 董玉 孙国园 朱小玲 周昊霏 盛华 胡密霞 XIN Sha-sha;DU Yin-fei;DONG Yu;SUN Guo-yuan;ZHU Xiao-ling;ZHOU Hao-fei;SHENG Hua;HU Ni-xia(College of Pharmacy,Inner Mongolia Medical University,Hohhot 010110,China;Department of Pharmacy,Inner Mongolia International Mongolian Hospital,Hohhot 010020,China)

机构地区：[1]内蒙古医科大学药学院,呼和浩特市010110 [2]内蒙古自治区国际蒙医医院,呼和浩特010020

出　　处：《药物分析杂志》2018年第10期1817-1823,共7页Chinese Journal of Pharmaceutical Analysis

基　　金：2016年内蒙古自治区科技创新引导奖励基金;2015内蒙古自治区科技计划项目(kjt15sf20);内蒙古自治区高等学校"青年科技英才支持计划"青年科技领军人才(NJYT-14-A12)

摘　　要：目的:建立采用HPLC同时测定蒙药复方述达格-4中1-苯基-7-(3-甲氧基-4-羟基)苯基-5-醇-3-庚酮、高良姜素、山柰素、高良姜素-3-甲醚、α-细辛醚、木香烃内酯含量的方法。方法:采用C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.2%乙酸水溶液为流动相,梯度洗脱,流速0.6 mL·min^(-1),检测波长254 nm,柱温30℃,进样量为10μL。结果:1-苯基-7-(3-甲氧基-4-羟基)苯基-5-醇-3-庚酮、高良姜素、山柰素、高良姜素-3-甲醚、α-细辛醚、木香烃内酯的进样量与其峰面积具有良好的线性关系(r=0.999 9);平均加样回收率(n=6)为96.6%～101.1%,RSD为0.70%～1.8%;测定样品6批,1-苯基-7-(3-甲氧基-4-羟基)苯基-5-醇-3-庚酮、高良姜素、山柰素、高良姜素-3-甲醚、α-细辛醚、木香烃内酯的平均含量分别为0.008 4%～0.060 0%、0.010 7%～0.061 6%、0.010 9%～0.086 5%、0.006 2%～0.066 6%、0.011 0%～0.078 1%、0.005 1%～0.060 6%。结论:该方法可用于蒙药复方述达格-4的质量控制,可为蒙药复方述达格-4资源开发利用提供理论依据。Objective：To establish a method for simultaneous determination of 6 components including 1-phenyl-7-（3-methoxy-4-hydroxy） phenyl-5-ol-3-heptanone, galangin, kaempferide, galangin-3-methylether, alpha-asarone and costunolide in Mongolian medicine compound formula Shudage-4 by HPLC. Methods：Separation was performed on a C18 column（4.6 mm×250 mm, 5 μm） and the mobile phase consisted of acetonitrile-0.2% glacial acetic acid with gradient elution at a flow rate of 0.6 mL·min-1. The detection wavelength was 254 nm and the column temperature was 30℃. The injection volume was 10 μL. Results：1-phenyl-7-（3-methoxy-4-hydroxy） phenyl-5-ol-3-heptanone, galangin, kaempferide, galangin-3-methylether, alpha-asarone and costunolide exhibited good linear relationship with correlation coefficients of 0.999 9, the recoveries were 96.6%-101.1%, and the RSD were 0.70%-1.8%. The content ranges of the above mentioned components in 6 samples were 0.008 4%-0.060 0%, 0.010 7%-0.061 6%, 0.010 9%-0.086 5%, 0.006 2%-0.066 6%, 0.011 0%-0.078 1% and 0.005 1%-0.060 6%, respectively. Conclusion：The method can be applied in quality control of Mongolian medicine compound formula Shudage-4. The study can provide theoretical basis for development and utilization of Mongolian medicine Shudage-4.

关 键 词：蒙药复方述达格-4 菖蒲四味 民族药 1-苯基-7-(3-甲氧基-4-羟基)苯基-5-醇-3-庚酮 高良姜素 山柰素 高良姜素-3-甲醚 Α-细辛醚 木香烃内酯 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]