焦亚硫酸钠溶液浸泡对黄芪中黄酮类成分的影响  

Effect of Sodium Pyrosulfite Solution Soaking on Flavonoids in Astragali Radix

作　　者：金悦仙 朱乃亮[1] 赵丹[1,2] 王睿[1,3] 马国需 许旭东[1] Jin Yuexian;Zhu Nailiang;Zhao Dan;Wang Rui;Ma Guoxu;Xu Xudong(Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China;Jilin Agricultural University;Ludong University)

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]吉林农业大学 [3]鲁东大学

出　　处：《中国药师》2018年第10期1747-1750,共4页China Pharmacist

基　　金：中国医学科学院医学与健康科技创新工程协和重大协同创新项目(编号:2017-I2M-1-013)

摘　　要：目的:考察焦亚硫酸钠溶液浸泡前后黄芪中黄酮类成分的变化,确定焦亚硫酸钠溶液浸泡对黄芪药材质量的影响,为评价焦亚硫酸钠溶液浸泡加工对黄芪质量的影响提供依据。方法:对收集的黄芪进行焦亚硫酸钠溶液浸泡加工,采用HPLC-DAD方法,建立黄芪中4种黄酮类成分的含量测定方法。结果:黄芪中毛蕊异黄酮葡萄糖苷、芒柄花苷、芒柄花素、紫檀芪线性范围分别为0. 048～60. 000μg·ml^(-1)、0. 019～30. 000μg·ml^(-1)、0. 019～40. 000μg·ml^(-1)、0. 019～40. 000μg·ml^(-1)。焦亚硫酸钠浸泡过的黄芪和正常组相比,毛蕊异黄酮葡萄糖苷和芒柄花苷类成分均显著下降,并且随着焦亚硫酸钠浓度的增大和浸泡时间的增加,含量降低趋势明显,高浓度组相差2～3倍左右;焦亚硫酸钠浸泡对芒柄花素成分的影响相对较小,但紫檀芪高浓度浸泡组与正常组的含量可差10倍左右。结论:焦亚硫酸钠处理对黄芪药材的化学品质具有一定程度的负面影响,特别是对有效成分具有显著的降低作用,因此,采用焦亚硫酸钠溶液浸泡黄芪来达到保鲜、防蛀、延长保质期的作法不可取。Objective： To analyze the contents of flavonoids in Astragali Radix before and after sodium pyrosulfite solution soaking,so as to provide reliable methods for scientifically evaluating and effectively controlling the quality of Astragali Radix. Methods：After sodium pyrosulfite solution soaking,the contents of calycosin glucoside,ononin,formononetin and pterostilbene were analyzed by HPLC-DAD. Results： Calycosin glucoside,ononin,formononetin and pterostilbene showed a good linear relationship within the range of 0. 048-60. 000 μg·ml^-1,0. 019-30. 000 μg·ml^-1,0. 019-40. 000 μg·ml^-1 and 0. 019-40. 00 0μg·ml^-1,respectively. After sodium pyrosulfite solution soaking,the contents of calycosin glucoside and ononin decreased,furthermore,with the increase of sodium sulfite solution concentration and soaking time,the decrease trend was more obvious,and the differences between the high concentration group and the normal group were 2-3 times. The content of formononetin was essentially the same,while the difference in pterostilbene content between the high concentration groups and the normal group was 10 times. Conclusion： After sodium pyrosulfite solution soaking,the contents of flavonoid glycoside components are reduced,therefore,it is not advisable to use sodium pyrosulfite solution soaking to achieve keeping fresh,mothproof and prolonging the shelf life of Astragali Radix.

关 键 词：黄芪 焦亚硫酸钠处理 黄酮类成分 含量 

分 类 号：R283.1[医药卫生—中药学]