肝组织中四种新型安眠药的固相萃取-气相色谱检测法  

Determination of four kinds of novel hypnotic drugs in liver sample by solid-phase extraction gas-chromatography

作　　者：舒翠霞[1] 邓轲[2] 张蕾萍[3] 董颖[3] 华炜婕 赵璟悠 Shu Cuixia;Deng Ke;Zhang Leiping;Dong Ying;Hua Weijie;Zhao Jinyou(Suzhou Public Security Bureau,Su zhou,Jiangsu215131,China;Deyang Public Security Bureau,Deyang,Sichuan,618000,China;Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China)

机构地区：[1]苏州市公安局刑侦支队,江苏苏州215131 [2]德阳市公安局刑侦支队,四川德阳618000 [3]公安部物证鉴定中心,北京100038

出　　处：《中国法医学杂志》2018年第5期506-509,共4页Chinese Journal of Forensic Medicine

摘　　要：目的建立固相萃取-气相色谱法检测肝组织中四种新型安眠药。方法将剪碎的肝组织样品用6mL pH7.4的磷酸二氢钾/磷酸氢二钠缓冲液稀释,振荡离心后取上层清液过HLB固相萃取柱,异丙醇/二氯甲烷洗脱,收集洗脱液,浓缩后供GC/NPD检测。结果利用该方法对添加佐匹克隆、褪黑素、唑吡坦和扎来普隆四种新型安眠药物的肝组织进行固相萃取和检测,萃取净化效果好,四种药物之间能很好分离,药物浓度在0.05～5μg/g范围内线性关系良好,定量下限为0.05μg/g,低、中、高三浓度的回收率均在80%左右,日内、日间精密度均较稳定。结论该方法可有效的同时检测肝组织中四种新型安眠药物。Objective To establish a method for determination of four kinds of novel hypnotic drugs in liver sample by solid -phase extraction gas-chromatography. Methods We extracted the target drugs from liver sample by solid-phase extraction and detected them with GC/NPD. The SPE column was rinsed sequentially with deionized water and 0.5%ammonia in methanol-water, and then eluted with dichloromethane- propanol. Results The target drugs can be separated well from each other by the proposed method without the endogenous interference. The linear rang was from 0.05 to 5μg/g with the correlation coefficients of 0.997 for zopiclone and zaleplon, 0.991 for melatonin and 0.995 for zolpidem. The inter-day and intra-day precisions of target drugs are less than 13.9%. Conclusion The method has high sensitivity and good reproducibility without the endogenous interference. The result is accurate and reliable with a wide linear range. It can be used in forensic toxicological analysis .

关 键 词：新型安眠药物 肝组织 固相萃取 GC/NPD 

分 类 号：D919[医药卫生—法医学]