IL-27通过STAT1信号转导途径诱导HepG2.2.15细胞表达MxA发挥抗病毒效应  

Antiviral effects of IL-27 on HBV in HepG2. 2. 15 cells via inducing MxA by STAT1 signal transduction pathway

作　　者：潘颖 闫波[1] 王传博[2] 汪洪杰[1] 王齐[1] 刘晓燕[1] PAN Ying;YAN Bo;WANG Chuanbo;WANG Hongjie;WANG Qi;LIU Xiaoyan(Anhui Medical College,2The Second Affiliated Hospital of Anhui Medical University,Hefei 230601,China)

机构地区：[1]安徽医学高等专科学校 [2]安徽医科大学第二附属医院,安徽合肥230601

出　　处：《细胞与分子免疫学杂志》2018年第6期511-516,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81202808);安徽医科大学校基金(2013xkj028)

摘　　要：目的研究白细胞介素27(IL-27)对Hep G2. 2. 15细胞乙型肝炎病毒(HBV)复制、抗原分泌的影响及其机制。方法以(0、20、50) ng/m L IL-27处理Hep G2. 2. 15细胞,采用荧光定量PCR检测细胞上清液HBV-DNA水平,ELISA检测细胞上清液HBV表面抗原(Hbs Ag)和HBV e抗原(HBe Ag)含量;免疫细胞化学染色法检测细胞HBV核心抗原(HBc Ag)的表达和定位,Western blot法检测细胞信号转导子与转录激活子1(STAT1)、磷酸化的STAT1(p-STAT1)、黏病毒抗性蛋白A(MxA)的蛋白水平。结果不同剂量IL-27处理后,Hep G2. 2. 15细胞上清中HBV-DNA、HBs Ag、HBe Ag及细胞内HBc Ag含量逐渐下降;随着IL-27剂量升高,细胞内p-STAT1表达水平显著上升,而STAT1表达未见明显变化;进一步研究发现,IL-27能诱导Hep G2. 2. 15细胞表达重要抗病毒蛋白Mx A,呈剂量和时间依赖性。结论 IL-27通过激活STAT1信号途径诱导Mx A表达发挥抗HBV活性。Objective To investigate the effect of interleukin 27（ IL-27） on hepatitis B virus（ HBV） replication and antigen secretion in Hep G2. 2. 15 cells and related mechanisms. Methods Hep G2. 2. 15 cells were treated with IL-27 at various doses. The levels of HBs Ag and HBe Ag in the supernatant of the cell cultures were measured by ELISA,and the levels of HBV DNA was detected by real-time quantitative PCR. The expression and localization of HBV core antigen（ HBc Ag） in Hep G2. 2. 15 cells were observed by immunocytochemical staining. Moreover,the expression of STAT1,phosphorylated STAT1（ p-STAT1） and myxovirus-resistance protein A（ Mx A） were analyzed by Western blotting. Results After the treatment with different doses of IL-27,the levels of HBV-DNA,HBs Ag,HBe Ag and intracellular HBc Ag in Hep G2. 2. 15 cells gradually decreased. With the increase of IL-27 dose,the expression level of p-STAT1 significantly increased. There was no significant change in STAT1 expression. Further studies also showed that IL-27 induced Mx A expression in a dose-and time-dependent manner in Hep G2. 2. 15 cells. Conclusion IL-27 could induces Mx A expression by activating STAT signaling pathway to exert anti-HBV activity.

关 键 词：肝炎病毒 乙型(HBV) 白细胞介素27(IL-27) 黏病毒抗性蛋白A(MxA) 

分 类 号：R392.11[医药卫生—免疫学] R373.21[医药卫生—基础医学]