超滤法测定天麻素与不同血浆的蛋白结合率  

Determination of protein binding rate of gastrodin with different plasma by ultrafifi ltration

作　　者：鲍慧玮[1] 徐阳 王萌 杨波[1] BAO Huiwei;XU Yang;WANG Meng;YANG Bo(College of Pharmacy,Changchun University of Chinese Medicine,Changchun 130117,China;Changchun Medical College,Changchun 130031,China)

机构地区：[1]长春中医药大学药学院,长春130117 [2]长春医学高等专科学校,长春130031

出　　处：《长春中医药大学学报》2018年第5期829-832,共4页Journal of Changchun University of Chinese Medicine

基　　金：国家自然科学基金青年基金项目(81403138);吉林省卫生计生委项目(2016Q052);吉林省中医药管理局项目(2017005)

摘　　要：目的建立测定天麻素与小鼠、大鼠、家兔的血浆蛋白结合率的实验方法,并计算天麻素与不同血浆的蛋白结合率。方法采用HPLC法测定天麻素的含量,Agilent TC-C18(250 mm×4.6 mm,5μm)色谱柱,流动相为甲醇:水(10:90,v/v),体积流量为1.0 mL/min,柱温为25℃,检测波长为220 nm。通过超滤法比较天麻素与小鼠、大鼠、家兔的血浆蛋白结合率。结果天麻素与小鼠血浆在5、20、50μg/m L浓度下的蛋白结合率为36.18%±0.54%、35.93%±0.48%、36.26%±0.25%;与大鼠血浆在5、20、50μg/m L浓度下的蛋白结合率为35.86%±0.27%、36.11%±0.36%、36.57%±0.39%;与家兔血浆在5、20、50μg/m L浓度下的蛋白结合率为37.00%±0.22%、36.90%±0.17%、37.15%±0.31%。结论天麻素与小鼠、大鼠、家兔血浆均属于低强度结合,无差异性和浓度依赖性。Objective To establish an experimental method for determining the plasma protein binding rate of gastrodin to mice, rats and rabbits, and the protein binding rate of gastrodin to different plasma was calculated.Methods The content of gastrodin was determined by HPLC on the Agilent TC-C18（250 mm×4.6 mm, 5 μm）column, the mobile phase was methanol： water（10：90, v/v）, the volume flow rate was 1.0 mL/min, the column temperature was 25, and the detection wavelength was 220 nm. The plasma protein binding rate of gastrodin to mice, rats and rabbits were compared by ultrafi ltration method. Results The protein binding rate of gastrodin to mouse plasma at 5, 20, 50 μg/mL were 36.18%±0.54%, 35.93%±0.48%, 36.26%±0.25%, respectively; and to rat plasma were 35.86%±0.27%, 36.11%±0.36%, 36.57%±0.39%, respectively; and to rabbit plasma were 37.00%±0.22%,35.90%±0.17%, 37.15%±0.31%, respectively. Conclusion The plasma of gastrodin in mice, rats and rabbits were low intensity binding, no difference and concentration dependence.

关 键 词：天麻素 超滤法 HPLC 血浆蛋白结合率 

分 类 号：R285.5[医药卫生—中药学]