梅花鹿茸Ⅰ型胶原对骨质疏松大鼠Wnt/β-catenin信号通路的影响  

Effect of sika deer antler collagen type Ⅰ on Wnt/β-catenin signaling pathway in osteoporotic rats

作　　者：石晓征[1] 李娜[1] 李晓华[1] 曲晓波[1] SHI Xiaozheng;LI Na;LI Xiaohua;QU Xiaobo(Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学,长春130117

出　　处：《长春中医药大学学报》2018年第5期832-835,共4页Journal of Changchun University of Chinese Medicine

基　　金：吉林省卫生计生委青年科技骨干培养计划项目(2016Q050)

摘　　要：目的研究梅花鹿茸I型胶原(SDC-I)对骨质疏松大鼠Wnt/β-Catenin信号通路的影响,探索SDC-I对骨质疏松大鼠的保护作用机制。方法应用去势法建立大鼠骨质疏松模型,随机分成空白组,模型组, SDC-I低、中、高剂量组(0.05、0.1、0.2 g/kg),灌胃给药90 d后取材。应用X线仪及骨骼强度仪检测大鼠股骨密度(BMD)和最大载荷量;采用ELISA法检测大鼠血清中血清骨钙素(BGP)和抗酒石酸酸性磷酸酶(TRACP)水平;应用Real Time PCR(RT-PCR)技术检测Wnt/β-Catenin信号通路中β-链蛋白(β-Catenin)、低密度脂蛋白受体相关蛋白5(LRP5)的mRNA表达水平。结果与模型组相比,SDC-I作用的各组大鼠BMD、最大载荷量显著增高;血清中BGP水平增高而TRACP水平减低;Wnt通路相关β-Catenin、LRP5的mRNA表达水平显著增高。结论 SDC-I可显著提高骨质疏松大鼠的骨密度,其保护机制与上调骨质疏松大鼠骨中β-Catenin、 LRP5的m RNA表达,激活Wnt/β-Catenin信号通路相关。Objective To observe the effect of SDC-I on classicl Wnt/β-Catenin signaling pathway, and to discuss the mechanism of on SDC-I osteoporosis rats model. Methods The osteoporosis rats model were established by castration method, the rats were randomly divided into 5 groups, namely blank group, model group, and lowdoes, medium-does and high-does（0.05, 0.1, 0.2 g/kg） SDC-I groups, 90 days after gavage. The bone mineral densities and maximum load of femur were measured by X-ray and bone strength instrument. BGP and TRACP levels were measured by ELISA assay. Quantitative analysis was made for the expressions of β-Catenin and LRP5 mRNA by RT-PCR. Results Compared with the model group, the BMD and maximum load of rats treated with SDC-I were significantly higher than those of the model group. Serum BGP level increases and TRACP level decreases. The mRNA expression level of Wnt pathway associated β-Cavities and LRP5 was signifi cantly increased. Conclusion Conclusion SDC-I can signifi cantly increase bone density in osteoporotic rats, and its protective mechanism is related to the up regulation of beta Catenin, mRNA expression of LRP5 and activation of Wnt/β-Catenin signaling pathway in osteoporotic rat bone.

关 键 词：梅花鹿茸Ⅰ型胶原 骨质疏松 WNT/Β-CATENIN信号通路 

分 类 号：R285.5[医药卫生—中药学]