白英提取物抑瘤有效成分的初步筛选  

Screening the anti-tumor active components of the Solanum lyratum Thunb.extract

作　　者：王卫芳[1,2] 刘悦 吴广谋[3] 杨唐 张迪 徐雅娟 WANG Weifang;LIU Yue;WU Guangmou;YANG Tang;ZHANG Di;XU Yajuan(Changchun University of Chinese Medicine,Changchun 130117,China;Jilin Province Academy of Traditional Chinese Medicine,Changchun 130012,China;Institute of Military Veterinary,Academy of Military Medical Sciences,Changchun 130122,China)

机构地区：[1]长春中医药大学,长春130117 [2]吉林省中医药科学院,长春130012 [3]军事医学研究院军事兽医研究所,长春130122

出　　处：《长春中医药大学学报》2018年第5期883-886,共4页Journal of Changchun University of Chinese Medicine

基　　金：吉林省科技厅自然科学基金研究项目(2016010 1341JC);吉林省大学生创新创业训练计划项目(201710199088);吉林省博士后科研资助项目(201410)

摘　　要：目的从分离白英水提液得到的不同组分中筛选抑瘤作用的主要有效成分,对不同肿瘤细胞体外增殖的抑制作用进行验证,为研究其诱导细胞凋亡机制奠定基础。方法选取人宫颈癌细胞He La、人胃癌细胞BGC-823细胞为研究对象,用阳性对照药(5-Fu)及白英不同组分进行干预。CCK-8法检测药物抑瘤率,并计算IC50值,比较组间药效强度。HE、AO/EB染色及镜下观察给予不同浓度药物处理时细胞数目、形态和细胞凋亡情况的变化。结果白英提取物不同组分均有一定的抗肿瘤作用,其中皂苷元的抑瘤作用最强,以中剂量(0.067 6 mg/mL)效果最好,对2种肿瘤细胞抑瘤率分别达到86.21%和88.92%。结论白英提取物具有抑制肿瘤细胞增殖的活性,能诱导肿瘤细胞凋亡,实验组与阳性对照组比较,差异无统计学意义。Objective To study the effective components of extract on proliferation inhibition and inducing apoptosis in different tumor cells in vitro, and lay a foundation for studying antitumor mechanism of Solanum lyratum Thunb.extract. Methods Selected Human gastric cancer cells BGC-823 and human cervical cancer cells HeLa as the subjects, and 5 fl uorouracil was the positive control drugs. The inhibition rate of drugs was detected by CCK-8, and the IC50 value was compared the effi cacy between groups. At the same time, use HE, AO/EB staining and microscopic observation showed changes in cell number, morphology and apoptosis in the treatment of different concentrations of drugs. Results Compared with control group, the proliferation inhibitory rate and apoptosis rate obviously. With the dose 0.0676 mg/ml, the two inhibitory rate of tumor cells was 86.21% and 88.92% respectively. Conclusion The extract from Solanum lyratum Thunb. can induce apoptosis and inhibit proliferation of Hhuman gastric cancer cells BGC-823 and human cervical cancer cells HeLa, and display effect in a dose-dependent. The experimental group and the positive control group have no signifi cant differences.

关 键 词：白英提取物 抑瘤作用 有效成分 

分 类 号：R284.2[医药卫生—中药学]