炭基复合材料生物相容性研究  

Study on biocompatibility of carbon-based composites

作　　者：殷延雄 于澍[1] 李云平[1] 吴强[1] 李晓[1] 钟慧[2] 邓幼文[3] 肖涛[3] 刘立宏[3] 郭晓柠[3] YIN Yanxiong;YU Shu;LI Yunping;WU Qiang;LI Xiao;ZHONG Hui;DENG Youwen;XIAO Tao;LIU Lihong;GUO Xiaoning(Key Laboratory for Powder Metallurgy,Central South University,Changsha 410083,P.R.China;School of Life Sciences,Central South University,Changsha 410013,P.R.China;Second Xiangya Hospital,Central South University,Changsha 410011,P.R.China)

机构地区：[1]中南大学粉末冶金国家重点实验室,长沙410083 [2]中南大学生命科学学院,长沙410013 [3]中南大学湘雅二医院,长沙410011

出　　处：《生物医学工程学杂志》2018年第5期740-748,共9页Journal of Biomedical Engineering

基　　金：国家高技术研究发展计划(863计划)资助项目(2015AA033503);国家自然科学基金资助项目(31500091)

摘　　要：本文采用低压化学气相沉积(LPCVD)工艺在炭/炭复合材料(C/C)表面制备炭化硅(SiC)涂层以及二氧化硅(SiO2)涂层。通过C/C、C/C-SiC、C/C-SiO2这3种炭基复合材料的细胞毒性实验、细胞直接接触实验和细胞黏附实验,考察炭基复合材料的生物相容性。细胞毒性、细胞直接接触和细胞黏附实验结果均表明,3种材料的细胞毒性均合格,生物相容性良好。但材料脱落的颗粒对噻唑蓝(MTT)法评价细胞毒性的准确性干扰很大,颗粒脱落越多,对小鼠成纤维细胞(L929细胞)抑制生长的影响越明显。因此,如用MTT法进行细胞毒性评价,材料的浸提液需要过滤处理。材料表面颗粒脱落实验结果显示,72 h内3种材料表面颗粒脱落量为C/C-Si O2>C/C> C/C-SiC,脱落曲线呈偏峰现象,在8 h达到峰值,至36 h趋于平稳;滤液分析显示,3种材料与模拟体液(SBF)溶液之间无离子交换现象。本文对炭基复合材料生物相容性的研究结果或对其今后在临床领域的应用具有一定指导意义。Silicon carbide（SiC） film and silicon dioxide（SiO_2） film were deposited on the surface of carbon/carbon composite（C/C） by low pressure chemical vapor deposition（LPCVD）.The biocompatibility of the three carbon-based composites,e.g.C/C,C/C-SiC,C/C-SiO_2 were investigated by cytotoxicity test,cell direct contact and cell adhesion experiments.Cytotoxicity,cell direct contact and cell adhesion showed that the three materials had no toxic effect on mouse fibroblasts（L929 cells）.However,the particles dropped off from the three materials had a great impact on evaluation accuracy of the thiazolyl blue（MTT） test.More the particles were lost,more growth inhibition to L929 cells.The evaluation accuracy of MTT method can be kept with the filtered extract of materials.Furthermore,the results of surface particles shedding experiment showed that the amount of surface particles shed from C/C-SiO_2 was the most,followed by C/C and C/C-SiC in 72 hours.Particles shedding curves showed there was a peak reached at eighth hour and then declined to the thirty-sixth hour.The filtrate analysis showed that there was no ion exchange between the three materials and simulated body fluid（SBF） solution.The results of this study on biocompatibility of carbon-based composites have certain guiding significance for their future application in clinical filed.

关 键 词：炭基复合材料 生物相容性 颗粒脱落 

分 类 号：R318.08[医药卫生—生物医学工程]