蒿甲醚在db/db小鼠中抗糖尿病及抗肥胖的作用  

Anti-Diabetic and Anti-Obesity Effects of Artemether in db/db Mice

作　　者：姜宏卫[1,2] 辛亚凯 郭宇[1,2] 付留俊 刘婕[1,2] 李利平[1,2] 马瑜瑾[1,2] 彭慧芳[1,2] 范梦琳 符玮[1,2] 白金磊 陈香美[3] J IANG Hong-wei;XlN Ya-kai;GUO Yu;FU Liu-jun;LIU Jie;LI Li-ping;MA Yu-jin;PENG Hui-fang;FAN Meng-lin;FU Wei;Bai Jin-lei;CHEN Xiang-mei(The First Affiliated Hospital,and College of Clinical Medicine of Henan University of Science and Technology,Luoyang 471003;Academican Workstation for Diabetic Kidney Disease Research of Henan Province,Luoyang 471003;Department of Nephrology,Chinese PLA General Hospital,Chinese PLA Institute of Nephrology,State Key Laboratory of Kidney Diseases,National Clinical Research Center for Kidney Diseases,Beijing 100853)

机构地区：[1]河南科技大学临床医学院,河南科技大学第一附属医院,洛阳471003 [2]河南科技大学第一附属医院糖尿病肾病研究院士工作站,洛阳471003 [3]中国人民解放军总医院肾脏病科,解放军肾脏病研究所,肾脏疾病国家重点实验室,国家慢性肾病临床医学研究中心,北京100853

出　　处：《中国中西医结合杂志》2018年第10期1209-1214,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.U1404805,No.81141059);河南科技大学研究生创新基金(No.CXJJ-2016-ZR15)

摘　　要：目的探索蒿甲醚在2型糖尿病模型db/db小鼠中的作用。方法 10只雄性C57BL/KsJ-db/db小鼠为糖尿病模型小鼠,用随机数字表法分为糖尿病组(1%甲基纤维素灌胃)及蒿甲醚组(1%甲基纤维素+蒿甲醚200 mg/kg灌胃),每组5只。另选5只雄性C57BL/KsJ-db/+小鼠为对照组(1%甲基纤维素灌胃),干预2周。每3天测定一次小鼠的摄食量、体重、空腹血糖。通过腹腔注射葡萄糖耐量试验(IPGTT)和胰岛素耐量试验(IPITT)及计算曲线下面积(AUC)来评估小鼠对葡萄糖的耐受力以及对胰岛素的敏感性,通过ELISA法测定血清胰岛素水平,并计算胰岛素抵抗指数(HOMEA-IR)评价小鼠的胰岛素抵抗水平。HE染色法观察小鼠胰腺和肝脏的形态。结果与糖尿病组比较,蒿甲醚组14天平均摄食量、空腹血糖明显降低(P <0. 05);与对照组比较,蒿甲醚组的体重增长率明显降低(P <0. 05)。与本组干预前比较,蒿甲醚组干预后IPGTT及IPITT的AUC明显降低(P <0. 05)。糖尿病组的HOMA-IR较对照组升高,同时蒿甲醚组的HOMA-IR低于糖尿病组(P <0. 05)。胰腺和肝脏HE染色结果显示蒿甲醚改善了db/db小鼠的胰岛空泡变性及肝脂肪变性。结论蒿甲醚明显改善了db/db小鼠的血糖稳态和胰岛素抵抗,并有预防肥胖,减轻脂肪肝的作用。Objective To investigate the effects of artemether on type 2 diabetic db/db mice. Methods Ten male C57 BL/KsJ-db/db mice were diabetic mice,which were divided into DM group（ 1% methylcellulose,intragastric administration） and artemether group（ 1% methylcellulose ＋ 200 mg/kg of artemether,intragastric administration） by random digital table method,5 in each group. Another 5 male C57 BL/KsJ-db/＋ mice were selected as control group（ 1% methylcellulose,intragastic administration） for 2 weeks. The treatment time was 2 weeks. The food intake,body weight and fasting blood glucose of mice were measured every 3 days. The intraperitoneal glucose tolerance test（ IPGTT） and insulin tolerance test（ IPITT） were performed to evaluate the tolerance to glucose and insulin sensitivity. The serum insulin levels were determined using ELISA kits,and insulin resistance index（ HOMEA-IR）were calculated to evaluate insulin resistance. HE staining was used to observe the morphology of pancreas and liverin mice. Results Compared with the DM group,the average food intake of 14 days and fasting glucose of artemether group significantly reduced（ P〈0. 05）. Compared with the control group,the weight increase rate of artemether group significantly reduced（ P〈0. 05）. Compared with pretreatment,the AUCs of IPGTT and IPITT of artemether group decreased significantly after the intervention（ P〈0. 05）.The HOMA-IR of DM group was higher than that of the control group,while the HOMA-IR of artemether group was lower than that of DM group（ P〈0. 05）. HE staining of pancreas and liver showed that artemether significantly ameliorated islet vacuolar degeneration and hepatic steatosis in db/db mice. Conclusion Artemether significantly improved glucose homeostasis and insulin resistance of db/db mice,and had the potential activity to prevent obesity,reduce the severity of fatty liver.

关 键 词：蒿甲醚 血糖稳态 胰岛素抵抗 脂肪肝 DB/DB小鼠 

分 类 号：R285.5[医药卫生—中药学]