microRNA-375通过下调PSEN1的表达诱导胰岛β细胞凋亡  

MicroRNA-375 Induces Islet IS-cells Apoptosis by Decreasing the Protein Level of PSEN1

作　　者：曹月 陆丹丹 常晓嫒 李雅婷[1] 朱云霞[1] CAO Yue;LU Dandan;CHANG Xiaoai;LI Yating;ZHU Yunxia(Jiangsu Province Key Lab of Human Functional Genomics,Nanjing Medical University,Nanjing,211166,China)

机构地区：[1]南京医科大学江苏省人类功能基因组学重点实验室,南京市211166

出　　处：《医学分子生物学杂志》2018年第5期268-272,共5页Journal of Medical Molecular Biology

基　　金：中国博士后科学基金面上资助一等资助（No.2016M590479）

摘　　要：目的研究miR-375能否诱导胰岛β细胞凋亡并对其机制进行初步探索。方法在大鼠胰岛β细胞系INS-1细胞中转染miR-375，运用流式细胞术检测INS-1细胞的凋亡情况。荧光素酶报告基因技术分析miR-375对Psenl的作用。Western印迹检测PSENl蛋白的表达。Hoechst染色检测干扰PSENl对INS-1细胞凋亡的影响。结果过表达miR-375诱导INS-1细胞凋亡；miR-375能够抑制Psenl基因3’UTR荧光素酶活性；过表达miR-375抑制PSENl蛋白的表达，干扰miR-375能够增加PSEN1蛋白的表达：干扰PSEN1的表达能够诱导INS-1细胞凋亡。结论miR-375通过下调PSENl的蛋白表达进而诱导胰岛β细胞凋亡。Objective To investigate whether miR-375 could induce apoptosis of pancreatic beta-cells and its mechanism. Methods The miR-375 mimics were transfected into INS-1 cells and the apoptosis of INS-1 was detected by flow cytometry. Luciferase reporter gene assay was used to detect the effect of miR-375 on Psenl3＇UTR. The protein level of PSEN1 was detected by Western blotting. By Hoechst staining, the effect of PSEN1 on INS-1 apoptosis was examined. Results Overexpression miR-375 could induce INS-1 apoptosis. MiR-375 could directly suppress the Luciferase ac tivity of Psenl3＇UTR and decrease the protein levels. Interfering miR-375 could restore the expression of PSEN1. Silencing PSEN1 could induce apoptosis of INS-1. Conclusion miR-375 induces pancreatic β-cell apoptosis by decreasing the protein level of PSEN1.

关 键 词：MICRORNA 凋亡 胰岛Β-细胞 

分 类 号：R34[医药卫生—基础医学]