氮硫共掺杂碳点为荧光探针检测姜黄素的研究  

Detection of Curcumin with Nitrogen-Sulfur Doped Carbon Dots as Fluorescence Probe

作　　者：弓辉[1] 李雪冰 吕俊杰[1] 王茹林[1] 卞伟[1] GONG Hui;LI Xue-bing;LU Jun-jie;WANG Ru-lin;BIAN Wei(School of Basic Medical Science,Shanxi Medical University,Taiyuan 030001)

机构地区：[1]山西医科大学基础医学院,山西太原030001

出　　处：《分析科学学报》2018年第5期633-638,共6页Journal of Analytical Science

基　　金：山西医科大学博士启动基金(No.03201514);山西医科大学科技创新基金(No.01201312);山西医科大学青年基金(No.022012010);山西医科大学大学生创新创业基金(No.20162202;20162203);山西省回国留学人员科研资助项目(No.2014-035)

摘　　要：本文以柠檬酸和L-半胱氨酸为原料,在微波加热的条件下合成了氮硫共掺杂碳点(NSCDs)。用透射电子显微镜、傅里叶变换红外光谱、紫外-可见吸收光谱、荧光光谱对合成的掺杂碳点进行了表征。NSCDs的荧光强度随着不同浓度姜黄素的加入而逐渐猝灭。实验考察了溶液pH、NSCDs浓度、反应时间对NSCDs-姜黄素体系荧光强度的影响,优化了实验条件,建立了一种快速灵敏的检测姜黄素含量的方法。最佳实验条件下,姜黄素的浓度在0.2~15μmoL/L的范围内与NSCDs的荧光强度成良好的线性关系,其检出限为62nmoL/L。将该方法应用于实际尿样和血样中姜黄素的测定,回收率在98.0%~102%之间。同时对NSCDs与姜黄素体系的荧光猝灭机理进行了初步的探讨,姜黄素与NSCDs的相互作用主要为静态猝灭过程。The nitrogen and sulfur doped carbon dots（NSCDs）were synthesized by using citric acid and L-cysteine as raw materials under microwave heating.The doped carbon dots were characterized by transmission electron microscopy（TEM）,fourier transform infrared（FT-IR）spectra,fluorescence spectra and ultraviolet-visible absorption spectra（UV-Vis）.The fluorescence intensity of NSCDs gradually quenched with the addition of curcumin.The effects of pH,NSCDs concentrations and reaction time on fluorescence intensity of NSCDs-curcumin system were investigated,and the experimental conditions were optimized.Under the optimal experimental conditions,the concentration of curcumin in the range of 0.2-15μmoL/L was in good linear relationship with the fluorescence intensity of NSCDs,with a detection limit of 62 nmoL/L.A fast and sensitive method for detecting curcumin was therefore established,and the method was applied to the determination of curcumin in urine samples and blood samples.The recoveries ranged from 98.0% to 102% and could be applied to the detection of real samples.At the same time,the fluorescence quenching mechanism between NSCDs and curcumin was discussed,and the results indicated that the interaction between curcumin and NSCDs was mainly static quenching.

关 键 词：氮硫共掺杂碳点 姜黄素 荧光探针 

分 类 号：O657.39[理学—分析化学]