不同手术入路构建肾血管性高血压大鼠模型的比较  

Comparison of different operative approaches of establishing renovascular hypertension rat models

作　　者：孙甲甲[1,2] 涂悦[2] 刘洋[2,3] SUN Jiajia;TU Yue;LIU Yang(Department of Gastroenteric-Thyroid-Mammary Surgery,Affiliated Hospital of Logistics College of PAP,Tianjin 300162,China;Brain Center,Affiliated Hospital of Logistics College of PAP Tianjin Key Laboratory of Neurotrauma Repair,Tianjin 300162,China;Department of Neurology,the Fourth People＇s Hospital of Shanghai City,Shanghai 200081,China)

机构地区：[1]武警后勤学院附属医院胃肠甲乳外科,天津300162 [2]武警后勤学院附属医院脑科中心天津市神经创伤修复重点实验室,天津300162 [3]上海市第四人民医院神经内科,上海200081

出　　处：《中国医药导报》2018年第29期13-16,共4页China Medical Herald

基　　金：国家自然科学基金青年科学基金项目(31200809)

摘　　要：目的比较经背部入路和经腹部入路构建肾血管性高血压大鼠模型的优缺点。方法将50只雌性SD大鼠按随机数字表法分为对照组(n=10)、腹部入路组(n=20)和背部入路组(n=20)。腹部入路组沿腹正中线取约2.5 cm纵行切口,背部入路组经双侧脊柱旁肾脏所在处取约1.5 cm纵行切口,两组大鼠均结扎双侧肾动脉后支,缝合后自由饮食饲养。比较两组血压水平的变化、手术时长、伤口是否被啃食、术后感染率和死亡率。结果腹部入路组及背部入路组大鼠术后3个月时血压均显著高于对照组(P <0.05);与腹部入路组比较,背部入路组大鼠手术时长较短,术后感染率较低,但被啃食例数较多,差异有统计学意义(P <0.05);术后3个月时背部入路组与腹部入路组大鼠死亡率比较,差异无统计学意义(P> 0.05)。结论两种入路构建肾血管性高血压大鼠模型各有特点,且术后的护理至关重要,需根据实际情况进行选择。Objective To compare the advantages and disadvantages of back approach and abdominal approach of es- tablishing renovascular hypertension rat models. Methods According to random number table method, 50 female SD rats were divided into control group （n = 10）, abdominal approach group （n = 20） and back approach group （n = 20）. Abdominal approach group was made an incision for about 2.5 cm along the abdominal medialline, back approach group was made two longitudinal incisions for about 1.5 cm along the bilateral paravertebral line where kidney located, both groups underwent ligation of posterior branches of bilateral renal arteries, with free diet after suture. The blood pressure, operation time, whether the wound was chewed, postoperative infection rate and mortality of the two groups were compared. Results After 3 months of operation, the blood pressure of abdominal approach group and back ap- proach group was signifcantly higher than that of control group （P 〈 0.05）. Compared with abdominal approach group, the operation time of back approach group was shorter, the postoperative infection rate was lower, while the number of cases being chewed was larger, the differences were statistically significant （P 〈 0.05）. After 3 months of operation, there was no significant difference on the mortality between abdominal approach group and back approach group （P 〉 0.05）. Conclusion The two approaches of establishing renovascular hypertension rat models have their own characteristics, and the postoperative nursing care is very important. It is necessary to choose the approach according to the actual conditions.

关 键 词：肾血管性高血压 肾动脉 大鼠模型 

分 类 号：R615[医药卫生—外科学]