急进高原后人外周血红细胞形态及血管内皮功能变化分析  

Analysis of the changes of erythrocyte morphology and vascular endothelial function in human peripheral blood cells after rushing into plateau

作　　者：王泽军 刘运胜 谭敏 薛锐 WANG Zejun;LIU Yunsheng;TAN Min;XUE Rui(Medical Teaching and Research Office of Cold Region,Department of Plateau Military Medicine,Army Medical University,Chongqing 400038,China;Medical Research Office of PLA Rocket Force,Scientific Research Institute,Army Medical University,Chongqing 400038,China;Dialysis Room,Department of Nephrology,Southwest Hospital of Army Medical University,Chongqing 400038,China;Cadet Brigade,Army Medical University,Chongqing 400038,China)

机构地区：[1]陆军军医大学高原军事医学系寒区医学教研室,重庆400038 [2]陆军军医大学科研学术处火箭军医学教研室,重庆400038 [3]陆军军医大学西南医院肾科透析室,重庆400038 [4]陆军军医大学学员旅,重庆400038

出　　处：《中国医药导报》2018年第29期112-115,共4页China Medical Herald

基　　金：国家自然科学基金青年科学基金项目(41101093)

摘　　要：目的分析急进高原后人外周血红细胞形态及血管内皮功能变化,为急性高原病(AMS)的预防和治疗提供参考。方法于2015年8月～2017年10月选取重庆地区拟急进高原的195名健康男性受试者,抽取其急进高原前(海拔500 m)及急进高原后(海拔3500 m)外周血10 mL,对其红细胞形态、血管内皮功能进行检测。按照受试者AMS发生情况,将其分为发生组、未发生组,对两组受试者外周血红细胞形态、血管内皮功能进行比较。结果急进高原后,受试者正常形态红细胞数量、血清一氧化氮(NO)均下降,其血清内皮素1(ET-1)、血管内皮生长因子(VEGF)、血管性血友病因子(vWF)均上升,差异均有统计学意义(P <0.05)。共有48名受试者发生AMS,发生率为24.62%。急进高原后,两组正常形态红细胞数量均下降,血清ET-1均上升,发生组上述变化较未发生组更为明显,差异均有统计学意义(P <0.05);发生组NO低于急进高原前,VEGF、钙黏蛋白、vWF高于急进高原前,差异均有统计学意义(P <0.05)。结论急进高原后人外周血红细胞趋于畸形化改变并伴有血管内皮功能受损,上述生理改变的进展可能是AMS发生的重要原因。Objective To analyze the changes of erythrocyte morphology and vascular endothelial function in human peripheral blood after rushing into plateau, so as to provide references for prevention and treatment of acute mountain sickness （AMS）. Methods From August 2015 to October 2017, a total of 195 healthy male objects going to rush into plateau in Chongqing were selected and 10 mL of peripheral blood was extracted before rushing into plateau （altitude 500 m） and after rushing into plateau （altitude 3500 m） to detect their erythrocyte morphology and vascular endothelial function. According to the occurrence of AMS, they were divided into occurrence group and non-occurrence group. The erythrocyte morphology and vascular endothelial function of the two groups were compared. Results After rushing into plateau, the number of normal red blood ceils and serum nitric oxide （NO） of the objects decreased, the serum endothelin-1 （ET-1）, vascular endothelial growth factor （VEGF） and von Wiltebrand factor （vWF） increased, the differences were statistically significant （P 〈 0.05）. A total of 48 objects developed AMS, the incidence of which was 24.62%. After rushing into plateau, the number of normal forms of red blood cells in the two groups decreased, and the serum ET-1 increased, the above changes of occurrence group were more obvious than non-occurrence group, the differences were statistically significant （P 〈 0.05）. As for occurrence group, the NO was lower than that before rushing into plateau, and VEGF, calcium mucin and vWF were higher than those before rushing into plateau, the differences were statistically significant （P 〈 0.05）. Conclusion The peripheral blood erythrocytes tend to change deformities and the vascular endothelial function is impaired. The progress of these physiological changes may be an important cause of the occurrence of AMS.

关 键 词：急进高原 外周血 红细胞形态 血管内皮功能 

分 类 号：R82[医药卫生—临床医学]