细菌生物被膜检测与分析方法  

Detection and analysis methods of bacterial biofilm

作　　者：刘宝宝[1] 汪洋[1] 易力[2] 王慧芳 王瑜欣 宫胜龙 涂春田 LIU Bao-Bao;WANG Yang;YI Li;WANG Hui-Fang;WANG Yu-Xin;GONG Sheng-Long;TU Chun-Tian(College of Animal Science and Technology,Henan University of Science and Technology,Luoyang,Henan 471003,China;College of Life Science,Luoyang Normal University,Luoyang,Henan 471022,China)

机构地区：[1]河南科技大学动物科技学院畜禽分子病原与免疫学重点实验室,河南洛阳471003 [2]洛阳师范学院生命科学学院,河南洛阳471022

出　　处：《微生物学通报》2018年第10期2263-2270,共8页Microbiology China

基　　金：国家重点研发计划(2018YFD0500100);国家自然科学基金(31772761;31540095);河南省自然科学基金(182300410047;162300410067)~~

摘　　要：细菌生物被膜(Biofilm,BF)由物体表面集聚生长的微生物群落和自身分泌的胞外物质构成,是造成细菌产生多重耐药性的原因之一。可靠、简单和快速的BF检测方法有助于有效预防和治疗相关疾病。基于不同原理的检测与分析方法已广泛用于BF研究中,本文从生物学方法、物理方法和化学方法等方面对BF检测方法进行总结,重点阐述显微镜技术在BF检测中的应用。并介绍了近年来发展的拉曼光谱、质谱成像、MALDI-TOF-MS等新技术,同时比较其优点和局限性,以便研究者找到最合适和最新的研究方法。Bacterial biofilms（BF） are surface-attached communities of cells embedded in an extracellular polymeric matrix. The cells are composed of polymers produced by the microorganisms themselves. Biofilm is a cause of multidrug resistance. A simple, reliable, quick biofilm analysis method facilitates the effective prevention and treatment of infectious diseases. In recent years, numerous methods for analyzing biofilm based on various principles have been developed. In this review, common microbiological, physical, and chemical methods of biofilm analysis are summarized, with a focus on the application of microscopy methods. New technologies such as Raman spectroscopy, imaging mass spectroscopy, and matrix-assisted laser desorption ionization-time of flight analysis are being applied in biofilm research. Accordingly, this review highlights the advantages and limitations of several methods with a view to assisting scientists in determining the most appropriate and up-to-date methods for their continued research.

关 键 词：生物被膜 检测方法 显微镜技术 优点 局限性 

分 类 号：R446.5[医药卫生—诊断学] S852.61[医药卫生—临床医学]