基于UFLC-Triple TOF-MS/MS的壮药战骨化学成分分析  

Chemical constituents of Premna fulva Craib by UFLC-Triple TOF-MS/MS

作　　者：梁玉婷 王静宇 苏薇薇[1] 幸林广 姚宏亮 林桥辉 彭维[1] LIANG Yu-ting;WANG Jing-yu;SU Wei-wei;XING Lin-guang;YAO Hong-liang;LIN Qiao-hui;PENG Wei(Guangdong Engineering and Technology Research Center for Quality and Efficacy Re-evaluation of Post-marketed Chinese Medicine,Sun Yat-sen University,Guangzhou 510275;Guangxi Nanning Baihui Pharmaceutical Group Co.,Ltd,Nanning 530003)

机构地区：[1]中山大学广东省中药上市后质量与药效再评价工程技术研究中心,广州510275 [2]广西南宁百会药业集团有限公司,南宁530003

出　　处：《中南药学》2018年第10期1369-1373,共5页Central South Pharmacy

基　　金：中山大学成果转化项目(No.33000-18825004)

摘　　要：目的应用超快速高效液相色谱串联三重四级杆飞行时间质谱(UFLC-Triple TOF-MS/MS)对壮药战骨的化学成分进行分析。方法以Phenomenex Kinetex 2.6 C_(18) 100A(3.0 mm×150 mm,5μm)为色谱柱,乙腈-0.1%甲酸为流动相,梯度洗脱,流速0.3 mL·min^(-1),采用配有ESI电喷雾源的高分辨三重四级杆飞行时间质谱,分别在正、负模式下进行检测。结果根据正负模式下获得的一级和二级质谱数据,通过对照品、准确分子量、裂解碎片对照及参考相关文献,确证了8个化合物(红景天苷、儿茶素、维采宁-2、夏佛塔苷、维采宁-3、木犀草苷、柚皮素、芹菜素),指认了25个化合物(包括4个苯丙素类和21个黄酮类)。结论系统研究了壮药战骨的化学成分,阐明了战骨的化学物质基础。Objective To identify the chemical constituents in Premna fulva Craib by UFLC-Triple TOF-MS/MS. Methods Phenomenex Kinetex 2.6 C18 100 A（3.0 mm×150 mm, 5 μm） was used with acetonitrile and 0.1% formic acid solution as the mobile in gradient elution. The flow rate was 0.3 mL·min^-1. The detector was highresolution quadrupole time of flight mass spectrometry with ESI ion source, tested in both positive and negative mode. Results Based on the mass spectrometry and certification of reference substances, 8 compounds were identified： salidroside, catechin, vicenin-2, schaftoside, vicenin-3, luteoloside, naringenin, and apigenin. Moreover, 25 compounds were confirmed（4 phenylpropanoids and 21 flavonoids）. Conclusion The chemical constituents in Premna fulva Craib are studied systematically, which provides scientific foundation for further research.

关 键 词：战骨 UFLC-Triple TOF-MS/MS 化学成分 

分 类 号：R284[医药卫生—中药学]