Imaging resolution of biocatalytic activity using nanoscale scanning electrochemical microscopy  

作　　者：Jase M .Abad Alvaro Y. Tesio EmUiano Martinez-Perinan Felix Pariente Encamacion Lorenzo 

机构地区：[1]Departamento de Qufmica Analftica y Andlisis Instrumental, Universidad Aut6noma de Madrid, Cantoblanco, 28049 Madrid, Spain [2]Centro de Investigaci6n y Desarrollo en Materiales Avanzados y Almacenamiento de Energfa de Jujuy-CIDMEJu (CONICET-UniversidadNacional de Jujuy), Centro de Desarrollo Tecnol6gico General Savio, Av. Martijena s/n, 4612-Palpald, Jujuy, Argentina [3]Instituto Madrileno de Estudios Avanzados en Nanociencia (IMDEA-Nanociencia), Campus UAM, Cantoblanco, 28049 Madrid, Spain

出　　处：《Nano Research》2018年第8期4232-4244,共13页纳米研究（英文版）

摘　　要：Scanning electrochemical microscopy represents a powerful tool for electro（chemical） characterization of surfaces, but its applicability has been limited in most cases at microscale spatial resolution, and the greatest challenge has been the scaling down to the nanoscale for fabrication and the use of nanometer-sized tips. Here, Pt nanoelectrodes with nanometer electroactive area were fabricated and employed for imaging a distribution of gold nanoparticles （AuNPs） and bioelectrocatalytic activity of a redox-active enzyme immobilized on gold surfaces.Scanning electrochemical microscopy represents a powerful tool for electro（chemical） characterization of surfaces, but its applicability has been limited in most cases at microscale spatial resolution, and the greatest challenge has been the scaling down to the nanoscale for fabrication and the use of nanometer-sized tips. Here, Pt nanoelectrodes with nanometer electroactive area were fabricated and employed for imaging a distribution of gold nanoparticles （AuNPs） and bioelectrocatalytic activity of a redox-active enzyme immobilized on gold surfaces.

关 键 词：scanning electrochemicalmicroscopy gold nanoparticle redox enzyme NANOELECTRODE lactate oxidase 

分 类 号：TB852.1[一般工业技术—摄影技术] O625.463[理学—有机化学]