羟基红花黄色素A对血小板活化因子的拮抗作用  

ANTAGONISTIC EFFECT OF HYDROXYSAFFLOR YELLOW A ON THE PLATELET ACTIVATING FACTOR RECEPTOR

作　　者：臧宝霞[1] 金鸣[1] 司南[2] 张彦[2] 吴伟[1] 朴永哲[1] 

机构地区：[1]北京市心肺血管疾病研究所药理研究室,北京100029 [2]北京中医药大学中药学院,北京100029

出　　处：《药学学报》2002年第9期696-699,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金 ( 396 70 884 );北京市跨世纪科技人才工程及北京市高科技实验室课题资助项目

摘　　要：目的　观察羟基红花黄色素A(HSYA)对血小板活化因子 (PAF)的拮抗作用。方法　以放射受体结合试验观察HSYA抑制 [3 H]PAF与家兔洗涤血小板 (WRP)特异性结合的作用 ,以比浊法观察HSYA抑制PAF介导的WRP及兔多型核白细胞 (PMNs)聚集的作用。结果　HSYA可浓度依赖地抑制 1 ,2及 4nmol·L-1 [3 H]PAF与WRP受体的结合 ;HSYA抑制PAF介导的WRP及兔PMNs聚集 ,均具明显的量效关系 ,其IC50 分别为 0 99及 0 70mmol·L-1 。AIM To observe the antagonistic effect of hydroxysafflor yellow A (HSYA) on the plat elet activating factor (PAF). METHODS Washed rabbit platelet (WRP) aggregation and rabbit polymorphonuclear leukocytes (PMNs) aggregation induced by PAF were observed by turbidimetric assay in vit ro . The PAF receptor antagonistic effect of HSYA was investigated by radio lig and binding assay (RLBA). RESULTS In RLBA the specific binding inhibition effect of HSYA was found to be concentra tion dependent in three different [ 3H]PAF concentrations. In the experi ments, WRP aggregation and rabbit PMNs aggregation induced by PAF (9 55×10 -10 , 9 55×10 -6 mol·L -1 ) were both inhibited by HSYA in a co ncentration dependent manner in vitro . The IC 50 of HSYA to inhibit WR P and rabbit PMNs aggregation was 0 99 and 0 70 mmol·L -1 , respectiv ely. CONCLUSION The PAF receptor binding can be antagonized by HSYA.

关 键 词：拮抗作用 羟基红花黄色素A 血小板活化因子 红花 受体结合试验 血小板聚集 多型核白细胞聚集 

分 类 号：R285[医药卫生—中药学]