肿瘤坏死因子-α对外周血单个核细胞重编程获得的神经干细胞增殖、分化的影响  

Effects of tumor necrosis factor-αon proliferation and differentiation of human induced neural stem cells reprogrammed from peripheral blood mononuclear cells

作　　者：唐玺和 梁有明[2] 黄锐[2] 秦国文[2] 蓝胜勇[2] 范益民[1] Tang Xihe;Liang Youming;Huang Rui;Qin Guowen;Lan Shengyong;Fan Yimin(Neurosurgery Department,the First Clinical Medical College of Shanxi Medical University,Taiyuan 030001,China;Neurosurgery Department,the People＇s Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,China)

机构地区：[1]山西医科大学第一附属医院神经外科,太原030001 [2]广西壮族自治区人民医院神经外科,南宁530021

出　　处：《神经疾病与精神卫生》2018年第8期568-570,F0003,共4页Journal of Neuroscience and Mental Health

摘　　要：目的 观察肿瘤坏死因子（TNF）-α对人外周血细胞重编程获得的神经干细胞（iNSCs）在体外增殖、分化的影响.方法 将人外周血单个核细胞重编程获得神经干细胞,使用免疫组化技术,观察TNF-α对其增殖以及向神经元及少突胶质细胞方向分化的作用.结果 低剂量TNF-α可以促进iNSCs的增殖,并促进其向神经元及少突胶质细胞方向分化.高剂量TNF-α可以导致其凋亡.结论 不同剂量的TNF-α对人iNSCs作用不同,这对于人iNSCs的应用有重要意义.Objective To explore the effects of tumor necrosis factor （TNF）- α on proliferation and differentiation of human induced neural stem cells （iNSCs） reprogrammed from peripheral blood mononuclear cells. Methods The human peripheral blood mononculear cells were reprogrammed to obtain iNSCs. Immunohistochemieal technique was used to observe the proliferation of TNF-α and the direction of differentiation to neurons and oligodendrocytes. Results A low dose of TNF-α can promote iNSCs proliferation and differentiate to a oligodendrocyte fate. High dose of TNF-α can induced apoptosis of iNSCs. Conclusions Different dose of TNF- α has different effect on human iNSCs, which is important for human iNSCs application.

关 键 词：肿瘤坏死因子-Α 神经干细胞 体细胞重编程 增殖 分化 

分 类 号：R446.6[医药卫生—诊断学]