罗氏内异方对子宫内膜异位症模型大鼠E钙黏蛋白表达的影响  

Study on Luoshi-Neiyi prescription in the expression of E-cadherin in endometriosis model rats

作　　者：罗碧云 李莉[2] Luo Biyun;Li Li(Master＇s Degree in 2015 of Guangzhou Chinese Medicine University,Guangzhou 510405,China;the First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,China)

机构地区：[1]广州中医药大学,510405 [2]广州中医药大学第一附属医院二妇科,510405

出　　处：《国际中医中药杂志》2018年第10期955-958,共4页International Journal of Traditional Chinese Medicine

基　　金：广东省自然科学基金（2015A030313359）

摘　　要：目的探讨不同剂量罗氏内异方对子宫内膜异位症（endometriosis，EMT）模型大鼠E钙黏蛋白（E-cadherin。E-cad）表达的影响。方法将SD雌性大鼠按随机数字表法分为高剂量组、中剂量组、低剂量组、模型组、假手术组、空白组，每组15只。除假手术组及空白组外，其余各组腹腔注射大鼠子宫内膜碎片建立EMT大鼠模型。造模成功后，低、中、高剂量组分别灌胃罗氏内异方11．88、23．76、47．52me,／100g。模型组、假手术组和空白组灌胃等体积生理盐水，连续灌胃8周。停药后，于妊娠第4天取各组大鼠左侧子宫，采用WB法检测E．cad表达。结果与模型组比较，中、高剂量组E-cad表达[（0．76±0．26）、（0．96±0．33）比（0．24±0．05）]升高（P〈0.05）；中、高剂量组组间比较差异无统计学意义（P〉0．05）。结论EMT大鼠E-cad呈现低表达，中、高剂量罗氏内异方可上调E-cad表达。Objective To investigate the effects of different doses Luoshi-Neiyi prescription in expression of E-cadherin of endometriosis model SD rats. Methods The female rats with SPF class SD were randomly divided into six groups： high-dose group, medium-dose group, low-dose group, model group, sham operation group and blank group, 15 of each group. Apart from the sham operation group and the blank group, the rats in the remaining group used abdominal cavity injection to establish the model of the endoheterosis rat model. After the successful establishment of the model, the mice were given high, medium,low dose and the sterilization water for 8 weeks. After stopping the drug, the left uterus of the rats was taken from the left uterus of each group in the fourth day of pregnancy to detect the E-cadherin by Western Blotting. Results Compared with the model group, the expression of E-cadherin （0.76 ±0.26, 0.96±0.33 vs. 0.24± 0.05） in the medium-dose and high dose groups significantly incresed （P〈0.05）. There was no differentence in the mediumdose and highdose groups （P〈0.05）. Conclusions The medium dose and high dose of Luoshi-Neiyi prescription can up-regulate the expression of E-cadherin in Endometriosis model rats.

关 键 词：子宫内膜异位症 E钙黏蛋白 罗氏内异方 大鼠 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]