白檀离体快繁技术  

Rapid Propagation of Symplocos paniculata In Vitro

作　　者：安佰义[1] 郭才南 包文慧 李凤飞[1] 赵赫[1] 陈丽[1] 安丰云 Baiyi Ant;Cainan Guo;Wenhui Bao;Fengfei Li;He Zhao;Li Chen;Fengyun An(College of Horticulture,Jilin Agricultural University,Changchun 130118,China;Yanbian Academy of Forestry,Yanji 133000,China)

机构地区：[1]吉林农业大学园艺学院,长春130118 [2]延边林业科学研究院,延吉133000

出　　处：《植物学报》2018年第5期693-699,共7页Chinese Bulletin of Botany

基　　金：长春市科技局项目(No.17DY014);吉林省教育厅项目(No.JJKH20180665KJ);吉林省科技厅重点科技攻关项目(No.20150-204045NY)

摘　　要：以白檀(Symplocos paniculata)幼嫩茎段为实验材料,通过对启动培养、增殖、生根培养及移栽的影响因子进行研究,初步建立了白檀的组织培养体系。结果表明:白檀外植体最适灭菌方案为0.1%升汞3分钟,无菌苗获得率达81%;最适初代启动培养基为1/2MS+30 g·L–1蔗糖+8 g·L–1琼脂,出芽率达86.83%;增殖最适培养基为1/2MS+1.0 mg·L^(-1) 6-BA+0.02mg·L^(-1) IBA+30 g·L–1蔗糖+8 g·L–1琼脂,增殖系数达3.57;最适生根培养基为WPM+0.5 mg·L^(-1) IBA+0.5 mg·L^(-1) NAA+20g·L–1蔗糖+2 g·L–1 AC+8 g·L–1琼脂,生根率达93%;炼苗后,移入园土:草炭土=1:1(v/v)的基质中,成活率达83%。With tender stem segments of Symplocos paniculata as the experiment material, the tissue culture system of S. paniculata was preliminarily established to research the key factors influencing initiation culture, proliferation, rooting culture and transplantation. Treatment with 0.1% HgCI2 for 3 min was the optimum scheme for explant sterilization of S. paniculata; survival rate of sterile seedlings was 81%. The rate of germination of initiation culture was 86.83% in the me- dium with 1/2MS＋30 g.L-1 sucrose＋8 g.L-1 agar. The proliferation coefficient was 3.57 in optimum culture medium with 1/2MS＋1.0 mg.L-1 6-BA＋0.02 mg.L-1 IBA＋30 g.L-1 sucrose＋8 g.L-1 agar. The suitable rooting medium was WPM＋0.5 mg.L-1 IBA＋0.5 mg.L-1 NAA＋20 g.L-1 sucrose＋2 g.L-1 AC＋8 g.L-1 agar, and rooting rate reached 93%. After being ac- climatized, the regenerated plants were transplanted into substrate of garden soil and peat soil （v：v=1：1）, with survival rate of regenerated plants of 83%.

关 键 词：白檀 外植体 组织培养 

分 类 号：S682.31[农业科学—观赏园艺]