人过氧化物氧还蛋白的生物信息学分析  

Bioinformatics Analysis of Human Peroxiredoxin

作　　者：杨莹莹 赵南晰[1] 安丽萍[1] 孙晶波[1] 郭笑[1] Yang Yingying;Zhao Nanxi;An Liping;Sun Jingbo;Guo Xiao(College of Pharmacy,Beihua University,Jilin 132013,China)

机构地区：[1]北华大学药学院,吉林吉林132013

出　　处：《北华大学学报（自然科学版）》2018年第6期758-763,共6页Journal of Beihua University（Natural Science）

基　　金：吉林省科技厅青年基金项目(20170520033JH;20180520051JH);吉林省科技计划项目(201703110399YY);吉林省中医药管理局项目(2017084);吉林省科学技术研究项目(JJKH20170066KJ)

摘　　要：目的分析6种人Prx蛋白的生物学信息.方法利用在线分析软件分析6种人Prx的蛋白质理化性质、信号肽、糖基化位点、磷酸化修饰、结构以及系统进化等,并对Prx1和Prx2的3D结构进行预测.结果 6种蛋白质间的理化性质存在较大差异;氨基酸序列中仅有Prx4信号肽是分泌蛋白,其他5个Prx成员均不是分泌蛋白;不同类型的Prx中所含糖基化以及潜在磷酸化位点数目不同;其氨基酸序列同源性约为30%,但高级结构高度保守;系统进化树可作为人Prx遗传分化研究的重要依据.结论掌握6种人Prx蛋白的理化性质,预测蛋白质潜在的特征,分析预测其结构以及通过序列比对掌握其亲缘关系,可为后续相关蛋白质功能及作用机制研究奠定良好的理论基础.该研究为深入开展Prx的酶学特征、功能研究及其在人体内抗氧化的分子机制研究提供理论依据.Objective This study used online analysis software to analyze the biological information of 6 humanPrx proteins. Method The online analytic software was used to analyze the protein physicochemical properties,signal peptide,glycosylation site,phosphorylation modification,structure and phylogenetic evolution of six humanPrx, and to predict 3D structures of Prx1 and Prx2. Results There were significant differences in thephysicochemical properties among six proteins. Only Prx4 containing signal peptides is a secreted protein,andother five members are not. The numbers of glycosylation sites and phosphorylated sites were different. Sequencealignment revealed that the overall amino acid sequence homology of them is about 30%. While 3D structures ofthem are highly conservative. Molecular evolution could be as an important basis for their studies of geneticdifferentiation. This study provided a theoretical basis for the enzymatic mechanisms, functional studies andmolecular mechanism of antioxidant in body. Conclusion Grasping the physicochemical properties of six humanPrx proteins, predicting the potential characteristics of proteins, analyzing and predicting their structure, andmastering their genetic relationship through sequence alignment,laid a good theoretical foundation for the study ofsubsequent related protein functions and mechanisms.

关 键 词：氧化物氧还蛋白 生物信息学 抗氧化 

分 类 号：Q51[生物学—生物化学] Q811.4