低温下外源腐胺对黑皮果蔗拔地拉光合性能的影响  

Effects of Putrescine Treatment on Photosynthesis in Chewing Cane(Badila) under Chilling Stress

作　　者：朱俊杰[1] 王天顺[2] 牙禹[2] 闫飞燕[2] ZHU Jun-jie;WANG Tian-shun;YA Yu;YAN Fei-yan(Guangxi University,Guangxi Nanning 530004,China;Agro-produets Quality Safety and Testing Technology Research Institute,Guan-gxi Academy of Agrieuhural Sciences,Guangxi Nanning 530007,China)

机构地区：[1]广西大学,广西南宁530004 [2]广西农业科学院农产品质量安全与检测技术研究所,广西南宁530007

出　　处：《西南农业学报》2018年第10期2069-2074,共6页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金(31460102);广西自然科学基金(2015GXNSFAA139082)

摘　　要：【目的】探讨外源腐胺对黑皮果蔗拔地拉(Saccharum officinarum L.cv.Badila)低温光合性能的影响,为果蔗低温育种和栽培措施的制定提供理论参考。【方法】采用低温培养箱处理的方法,对比分析外源腐胺叶面喷施结合低温处理和单独低温处理两种方式对拔地拉光合气体交换能力及叶绿素荧光效应与对照的差异。【结果】低温处理4 d后,和常温对照相比,除非光化学热耗散(NPQ)值外,其它光合气体交换参数值和叶绿素荧光参数值均显著下降,表明拔地拉是典型的冷敏感甘蔗类型。低温结合腐胺处理组和单独低温处理组相比,最大光化学效率Fv/Fm值、光响应曲线、Ci(胞间CO2浓度)响应曲线和CO2量子效率曲线值表现均升高,但NPQ响应曲线和电子传递量子效率曲线却表现相反,表明在低温下腐胺对拔地拉的效应主要与维持CO2同化相关,而对通过电子传递的能量耗散和非光化学途径的光能耗散没有明显作用。解除低温在常温下恢复4 d后,结合腐胺处理组除NPQ值之外,其余测定参数值都好于单独低温处理组,前者除了提供了更多光合产物外,极有可能还对光合机构结构的维持起重要作用。【结论】低温下外源腐胺处理对拔地拉光合碳同化的维持能力有明显效果,显著促进低温解除后光合性能的恢复,在果蔗生产中有潜在应用价值。【Objective】To provide a theoretical reference for the formulation of sugarcane breeding and cultivation measures at low temperature,the effects of putrescine treatment on the photosynthetic performance in a chewing cane（Saccharum officinarumL.cv.Badila） leaves under chilling stress were studied.【Method】The Badila seedlings were cultured in incubator and treated with chilling stress or putrescine treatment plus chilling stress,and the differences inphotosynthetic gas exchange andchlorophyll fluorescence parameters were compared with the control group.【Result】In contrast to the seedlings grown under normal temperature,except NPQ values,all the other tested photosynthetic parameter values were decreased obviously under chilling stress for 4 days,indicating the sensitive of photosynthesis to chilling in Badila seedlings.Compared with the chill treatment group,the 1 mM putrescine plus chill treatment group had better performance in Fv/Fm,light response curve,carbon dioxide response curve and carbon dioxide quantum efficiency response curve,whereas they were not good in NPQ response curves and electron transport quantum efficiency response curves.After 4 days recover under normal temperature,the 1 mM putrescine plus chill treatment group had obvious advantages in almost all of the tested parameters compared with the chill treatment group.This should be attributed to the higher efficiency in carbon assimilation and probably to the maintenance of the structure of the photosynthetic apparatus by the putrescine treatment under chilling condition.【Conclusion】The application of putrescine on Badila slowed down the decrease in photosynthetic carbon assimilation under chilling stress and significantly promoted the recovery of the photosynthetic apparatus from chilling stress.The study has potential apply values in chewing cane production.

关 键 词：多胺 拔地拉 冷害 光合性能 

分 类 号：S566.1[农业科学—作物学]