高丹草SRAP和SSR分子遗传连锁图谱的构建  

Construction of Genetic Linkage Map of Sorghum-Sudangrass Based on SRAP and SSR Molecular Markers

作　　者：石悦[1] 于肖夏[1] 南志标[2] 于卓[1] 吴国芳 杨东升[1] 张明飞[1] SHI Yue;YU Xiao-xia;NAN Zhi-biao;YU Zhuo;WU Guo-fang;YANG Dong-sheng;ZHANG Ming-fei(Agronomy College,Inner Mongolia Agricultural University,Hohhot 010019,China;College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730000,China)

机构地区：[1]内蒙古农业大学农学院,内蒙古呼和浩特010019 [2]兰州大学草地农业科技学院,甘肃兰州730000

出　　处：《中国草地学报》2018年第5期11-17,共7页Chinese Journal of Grassland

基　　金：国家自然科学基金面上项目(31472141);草地农业生态系统国家重点实验室项目(SKLGAE201614);国家"973"计划项目资助(2014CB138703)

摘　　要：为了定位高丹草低氢氰酸含量、产草量及抗逆性等重要性状的QTLs,以高丹草杂种F2为作图群体,利用SRAP和SSR两种分子标记技术对305个F_2分离单株及其亲本基因组DNA进行PCR扩增得到427个多态性分子标记位点(253个SRAP、174个SSR),采用Joinmap4.0作图软件构建了一张含SRAP和SSR两种标记的高丹草分子遗传连锁图谱。图谱有10个连锁群,各连锁群的长度变幅72.9cM^168.2cM,覆盖基因组总长度1273.4cM,含427分子标记,标记间的平均距离2.98cM。To identify the QTLs of hydrocyanic acid,high yield,strong stress resistance of SorghumSudangrass 427 polymorphic loci（253 SRAP and 174 SSR）were amplified with genomic DNA of 305 F2 individuals by SRAP and SSR molecular marker techniques.A genetic linkage map of Sorghum-Sudangrass was constructed based on SRAP and SSR molecular markers and Joinmap 4.0 software,consisting of 10 linkage groups and 427 markers,the length of each linkage group ranged from 72.9 cM to 168.2 cM,and the total length was 1273.4 cM,with an average distance at 2.98 cM.

关 键 词：高丹草 SRAP和SSR标记 遗传连锁图谱 

分 类 号：S541.1[农业科学—作物学]