不同碳氮钾源对香椿根际解磷菌溶磷效果的影响  

Influence of Different Carbon,Nitrogen and Potassium Sources on Phosphate Solubilization by Phosphate-solubilizing Bacteria From Toona sinensis Rhizosphere

作　　者：林英[1] 司春灿[1] 冯唐锴[1] 黄云祥 廖瑜璐 LIN Ying;SI Chuncan;FENG Tangkai;HUANG Yunxiang;LIAO Yulu(Department of Biological and Chemical Engineering,Jingdezhen University,Jingdezhen,Jiangxi 33300)

机构地区：[1]景德镇学院生物与化学工程系,江西景德镇333000

出　　处：《北方园艺》2018年第20期1-7,共7页Northern Horticulture

基　　金：江西省教育厅科学技术研究资助项目(GJJ151277;GJJ151279);景德镇市科技资助项目(2015NYZD020)

摘　　要：以香椿根际土壤为试验材料,采用稀释平板涂布法对香椿根际土样的解磷菌进行了分离,对选取的溶磷圈直径/菌落直径比值最大为2.73的解磷菌XCP9进行了16SrDNA分子鉴定,采用钼锑抗比色法,研究了不同碳源、氮源、钾源对XCP9溶解磷酸三钙效果的影响,以期为香椿微生物磷肥的开发提供一定的参考价值。结果表明:XCP9菌株与伯克氏菌(Burkholderiasp.)具有很高的同源性,不同碳氮钾源对XCP9的溶磷效果有极显著影响,且菌株XCP9的溶磷量与pH之间存在极显著负相关。当以甘露醇为碳源、硫酸铵为无机氮源、氯化钾为钾源时,菌株XCP9的溶磷能力达到最高为122.31μg·mL^(-1)。In order to provide some reference value for the development of microbial phosphate fertilizer for Toona sinensis.Rhizosphere soil of Toona sinensis was taken as test materials,phosphatesolubilizing bacteria were isolated fromToona sinensis rhizosphere using serial dilution plate method.The strain XCP9 performed maximum ratio of dissolving zone diameter to colony diameter were selected for 16 SrDNA molecular identification and the effect of different carbon,nitrogen and potassium sources on phosphate solubilization by XCP9 were studied.The results showed that the strain XCP9 had a very high homology with Burkholderiasp.,the phosphate solubilizing efficiencies of strain XCP9 among different carbon,nitrogen and potassium sources were very significant and there was a very strong significant negative correlation between phosphate solubilizing efficiencies and pH.When using mannitol as carbon source,ammonium sulfate as inorganic nitrogen source,potassium chloride as potassium source.Maximum P solubilization of strain XCP9 achieved with 122.31μg·mL^-1.

关 键 词：碳源 氮源 钾源 解磷菌 解磷能力 

分 类 号：S644.4[农业科学—蔬菜学]