耐盐紫花苜蓿SSR标记遗传多样性分析  

Genetic Diversity of SSR Markers in Salt Tolerant Alfalfa

作　　者：张喜斌[1] 麻冬梅 ZHANG Xibin;MA Dongmei(School of Life Science,Ningxia University,Yinchuan,Ningxia 750021;Western Ecological Research Center,Ningxia University,Yinchuan,Ningxia 750021)

机构地区：[1]宁夏大学生命科学学院,宁夏银川750021 [2]宁夏大学西部生态研究中心,宁夏银川750021

出　　处：《北方园艺》2018年第20期40-47,共8页Northern Horticulture

基　　金：宁夏回族自治区牧草育种专项资助项目(NYYZ-2014-Z12)

摘　　要：以5份不同耐盐紫花苜蓿为试材,利用5对SSR引物对其进行了遗传多样性检测,采用0/1法统计带型数据,并利用主成分分析和隶属函数法对5份紫花苜蓿的杂合度、香农指数、PIC、多态位点百分率、PNe/Ao综合评价。根据综合评价值(D值)对遗传变异大小进行排序,以期为后期遗传亲本的选择提供依据。结果表明:5个紫花苜蓿品种各位点各群体的平均DNA多态率为62.65%、有效等位基数平均数为3.66,低于等位基因观测值的平均数7.20、平均杂合度为0.736 6、平均PIC为0.689 0、平均香农指数为2.083;遗传变异程度大小为"巨能7号"(‘Juneng No.7’)>"抗旱15"(‘Kanghan 15’)>"巨能"(‘Juneng’)>"骑士T"(‘Qishi T’)>"阿迪娜"(‘Adina’);遗传距离为0.434 2~0.865 6;聚类分析表明"骑士T"/"阿迪娜"之间的遗传距离最小,为0.314 4,"巨能7号"/"抗旱15"之间的遗传距离最大,为0.725 0。Five different salt tolerant alfalfa varieties were used as materials for genetic diversity by 5 pairs of SSR primers,with statistical data by 0/1 method,and using principal component analysis and the method of membership function of 5 alfalfa heterozygosity,Shannon index,PIC,the percentage of polymorphic loci,PNe/Aocomprehensive evaluation.According to the comprehensive evaluation value（D value）,the size of genetic variation was sorted,in order to provide the basis for the selection of later genetic parents.The results showed that the average DNA polymorphism of five alfalfa varieties to each group,effective rate was 62.65%and the average was 3.66,lower than the average number of the number of alleles observed value of 7.20,the average heterozygosity was 0.736 6,the average PICwas0.689 0,the average Shannon-Wiener index was 2.083;the genetic variation was：‘Juneng 〉No.7＇‘Kanghan 15＇〉‘Juneng＇ 〉‘Qishi T＇〉 ‘Adina＇;genetic distance was 0.434 2-0.865 6;cluster analysis showed that the genetic distance between ‘Qishi T＇/‘Adina＇was minimum（0.314 4）,‘Juneng No.7＇/‘Kanghan 15＇had the furthest genetic distance（0.725 0）.

关 键 词：紫花苜蓿 遗传多样性 SSR 耐盐 

分 类 号：S551.7[农业科学—作物学]