白色金针菇液体发酵培养条件的优化  

Optimal Liquid Fermentation Conditions of White Flammulina velutipes

作　　者：王玉 黄亮 彭明梦 伊格禧 班立桐 WANG Yu;HUANG Liang;PENG Mingmeng;YI Gexi;BAN Litong(College of Agronomy ＆ Resources and Environment,Tianjin Agricultural University,Tianjin 300384;Tianjin Binhaidesheng Agricultural Technology Co.Ltd.,Tianjin 300300)

机构地区：[1]天津农学院农学与资源环境学院,天津300384 [2]天津滨海德盛农业科技有限公司,天津300300

出　　处：《北方园艺》2018年第20期148-153,共6页Northern Horticulture

基　　金：天津市农业科技成果转化与推广资助项目(201303010);天津市蔬菜产业技术体系创新团队资助项目(ITTVRS2017015)

摘　　要：以白色金针菇为试材,以菌丝体生物量、密度、萌发时间等为考察指标,采用单因素试验及L9(34)正交实验对影响其液体摇瓶培养时的条件因素(培养基的初始pH、接种龄、接种量、摇床转速)进行优化,以实现白色金针菇摇瓶液体菌种高密度发酵。结果表明:适宜的摇瓶培养条件为培养基初始pH 6.5,接种龄102h,接种量10%,摇床转速140r·min^(-1)。在上述条件下,金针菇菌丝体生物量达到17.93 g·L^(-1),密度104.21个·mL^(-1),直径1.35mm,萌发时间8h。White Flammulina velutipes was used as materil,the optimal culture conditions（medium initial pH,inoculum age,inoculum amount,shaker speed）were studied by single factor test and L_9（3-4）orthogonal test,indexes for mycelial biomass,density,germination time,in order to achieve the high density fermentation of white Flammulina velutipes.The results showed that the optimal culture conditions were the initial pH 6.5,inoculum age 102 hours,inoculum amount 10%,the shaking speed 140 r· min^-1.Under the conditions,the mycelial biomass of Flammulina velutipes reached17.93 g·L^-1,density was 104.21· mL^-1,the diameter was 1.35 mm,the germination time was 8 hours.

关 键 词：白色金针菇 液体发酵 培养条件 优化 

分 类 号：S646.15[农业科学—蔬菜学]