石菖蒲挥发油联合人参总皂苷通过调节PI3K/Akt/mTOR通路对痴呆模型APP/PS1双转基因小鼠的Aβ_(40)和GFAP的影响  

Effects of the co-administration of total ginsenoside and volatile oil of Acorus tatarinowii Schott on the levels of Aβ_(40) and GFAP in APP/PS1 double transgenic mice by adjusting the PI3K/Akt/mTOR pathway

作　　者：邓敏贞 黄丽平 马阮昕[4] 温晓文 Deng Minzhen;Huang Liping;Ma Ruanxin;Wen Xiaowen(Guangdong Provincial Hospital of Chinese Medicine,Guangdong Provincial Academy of Chinese Medical Sciences,Guangzhou 510120;The Second Clinical College,Guangzhou University of Chinese Medicine,Guangzhou 510120;2 Lingnan Normal University,Zhanjiang 524048,PR Chin;3.Hainan Medical University,Haikou 57119;4 The Sixth Affilited Hospital Of SUN Yatsen University,Guangzhou 510655;5 Zhongshan Hospital of Traditional Chinese Medicine,Zhongshan 52840)

机构地区：[1]广东省中医院/广东省中医药科学院/广州中医药大学第二临床医学院,广州510120 [2]岭南师范学院,湛江524048 [3]海南医学院,海口571199 [4]中山大学附属第六医院药学部,广州510655 [5]中山市中医院,中山528400

出　　处：《中药药理与临床》2018年第4期92-95,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：广东省中医药局科研基金项目(20181114);海南省自然科学基金(20168266);海南省科协青年科技人才学术创新计划项目(HAST201635);海南医学院科研培育基金项目(HY2015-01);2015年学校创新计划-优秀博士论文培养项目(A1-AFD018161Z01024)

摘　　要：目的:研究石菖蒲挥发油联合人参总皂苷对APP/PS1双转基因小鼠的Aβ_(40)和GFAP的含量变化及对调节PI3K/Akt/mTOR通路的影响。方法:112只APP/PS1双转基因小鼠随机分为8组,模型组、人参总皂苷75 mg/kg组、石菖蒲挥发油15 mg/kg组、石菖蒲挥发油15 mg/kg+人参总皂苷75 mg/kg、石菖蒲挥发油30 mg/kg+人参总皂苷150 mg/kg、石菖蒲挥发油60 mg/kg+人参总皂苷300 mg/kg、雷帕霉素0. 5 mg/kg组和LY294002 0. 75 mg/kg组。正常组和模型组灌胃等体积的生理盐水,各组连续给药30 d。末次给药后采用免疫组化检测GFAP; ELISA检测Aβ_(40)和GFAP; Western blot检测p-mTOR和p-Akt。结果:与正常组比较,模型组的Aβ_(40)和GFAP表达显著增加,p-mTOR和p-Akt表达显著减少。与模型组比较,人参总皂苷组、石菖蒲挥发油组和联合各剂量组的的Aβ_(40)和GFAP表达都显著减少,雷帕霉素组的p-mTOR表达显著减少及p-Akt的表达显著增加,LY294002组的p-mTOR表达显著增加及p-Akt的表达显著减少,联合各剂量组的p-mTOR和p-Akt的表达显著增加。与人参总皂苷组和石菖蒲挥发油组比较,其中联合各剂量组的Aβ_(40)和GFAP表达都显著减少。与LY294002组比较,雷帕霉素组和联合各剂量组的p-mTOR表达显著减少及p-Akt的表达显著增加。与雷帕霉素组比较,联合各剂量组的p-mTOR表达显著增加及p-Akt的表达显著减少。结论:石菖蒲挥发油联合人参总皂苷通过上调PI3K/Akt/mTOR通路来减少APP/PS1双转基因小鼠中Aβ_(40)和GFAP表达。Objective： To observe the effects of the co-administration of total ginsenoside and volatile oil of Acorus tatarinowii Schott on the levels of Aβ40 and GFAP in APP/PS1 double transgenic mice by adjusting the PI3 K/Akt/mTOR pathway. Methods： 112 APP/PS1 double transgenic mice were randomly divided into model group,total ginsenoside group（75 mg/kg）,volatile oil of Acorus tatarinowii Schott group（15 mg/kg）,Rapamine group（0. 5 mg/kg）,LY294002 group（0. 75 mg/kg） and co-administration of total ginsenoside and volatile oil of Acorus tatarinowii Schott group（co-administered group,the doses of volatile oil of Acorus tatarinowii Schott and total ginsenoside were 15 mg/kg and 75 mg/kg,30 mg/kg and 150 mg/kg,60 mg/kg and 300 mg/kg respectively）. Mice in the treated groups were administration with the corresponding drug form the 30 th day. Senile plaque on GFAP expression was observed by immunohistochemistry. Aβ 40 and GFAP were detected by ELISA. The expression of p-mTOR and p-Akt were detected by western blot. Results： As compared with the normal group,the expression of Aβ40 and GFAP were significantly increased（P 〈 0. 01）,but p-mTOR and p-Akt expression were significantly reduced in model group（P〈 0. 01）. As compared with the model group,the expression of Aβ40 and GFAP were significantly decreased in total ginsenoside group,volatile oil of Acorus tatarinowii Schott group and co-administered group（P 〈 0. 05）; p-mTOR expression was significantly decreased,but p-Akt expression was significantly increased in Rapamine group（P 〈 0. 05）; p-mTOR expression was significantly increased,but p-Akt expression was significantly decreased in LY294002 group（P 〈 0. 01）; p-mTOR and p-Akt expression were significantly increased in co-administered group（P 〈 0. 05）. As compared with the total ginsenoside group and volatile oil of Acorus tatarinowii Schott group,the expression of Aβ40 and GFAP were significantly decreased in co-administered group（P 〈 0. 05）. As compared wi

关 键 词：石菖蒲挥发油 人参总皂苷 阿尔兹海默病 PI3K/Akt/mTOR通路 

分 类 号：R285.5[医药卫生—中药学]