利用CRISPR/Cas9技术有效沉默菊花DgGA20ox基因  

DgGA20ox was effectively silenced by CRISPR/Cas9 technology in Chrysanthemum

作　　者：李翠[1,2] 郝福顺 孙立荣[1] LI Cui;HAO Fushun;SUN Lirong(Henan Key Laboratory of Plant Stress Biology,College of Life Science,Henan University,Kaifeng475004,China;Zhengzhou Professional Technical Institute of Electronic ＆ Information,Zhengzhou451450,China)

机构地区：[1]河南大学植物逆境生物学重点实验室/生命科学学院,河南开封475004 [2]郑州电子信息职业技术学院,河南郑州451450

出　　处：《河南科技学院学报（自然科学版）》2018年第5期22-26,共5页Journal of Henan Institute of Science and Technology(Natural Science Edition)

基　　金：国家自然科学基金(31070239);河南省教育厅科学技术研究重点项目(14B180030)

摘　　要：以菊花品种神马"Sma"为材料,利用CRISPR/Cas9技术编辑其赤霉素合成关键酶GA20氧化酶基因DgGA20ox.采用农杆菌介导的遗传转化方法,成功获得了沉默DgGA20ox菊花转基因植株.与对照相比,转基因植株变得矮小,茎节间显著缩短.测序结果显示,DgGA20ox基因发生了移码突变,说明利用CRISPR/Cas9技术能有效沉默菊花基因,这为开展菊花的基因工程研究奠定了基础.DgGA20ox encoding gene,which plays a key role in biosynthesis of gibberellins （GAs）,was edited usingCRISPR/Cas9 technology in chrysanthemum species “Sma”.Agrobacterium-mediated genetic transformation strategywas applied,and several transgenic plants expressinga DgGA20ox editing vector were generated in Chrysanthemum.Compared with the control,the transgenic plants were clearly short,and had shortened internodes.DNA sequencingresults showed that frame shift mutations occurred within DgGA20ox gene in Chrysanthemum transgenic plants.Thesefindings suggest that CRISPR/Cas9 is a power tool for effectively silencing genes in Chrysanthemum.This will paveaway for genetic engineering in chrysanthemum.

关 键 词：菊花 CRISPR/Cas9 基因编辑 DgGA20ox 

分 类 号：S682.11[农业科学—观赏园艺]