HPLC法检测萘普生注射液含量方法的建立  

Establishment of HPLC method for determination of naproxen injection

作　　者：张雨桐 张永红[1] 李志军 杜继红[3] 王建舫[1] 李佳[1] 崔德凤[1] 孙志文[3] ZHANG Yutong;ZHANG Yonghong;LI Zhijun;DU Jihong;WANG Jianfang;LI Jia;CUI Defeng;SUN Zhiwen(Beijing Key Laboratory of Traditional Chinese Veterinary Medicine;National Demonstration Center for Experimental Animal Educatio;College of Animal Science and Technology,Beijing University of Agriculture,Beijing 102206,Chin;2.Beijing Animal Disease Prevention and Control Center,Beijing 102600,Chin;3.Beijing Veterinary Drug Monitoring Institute,Beijing 102600,China)

机构地区：[1]兽医学(中医药)北京市重点实验室动物类国家级实验教学示范中心北京农学院动物科学技术学院,北京102206 [2]北京市动物疫病预防控制中心,北京102600 [3]北京市兽药监察所,北京102600

出　　处：《北京农学院学报》2018年第4期68-71,共4页Journal of Beijing University of Agriculture

基　　金：2018年度北京市农委新型生产经营主体科技能力提升项目;2017年度实验技术体系研发基金

摘　　要：【目的】探明高效液相色谱法(HPLC)法测定萘普生含量的可靠性。【方法】高效液相色谱法:流动相由甲醇∶0.01mol/L KH2PO4 (75∶25,V/V)组成;流速1.0m L/min;检测波长为272nm。【结果】HPLC法的平均回收率为99.08%,相对标准偏差(RSD)0.5%小于标准2%,线性方程为Y=4.326 9+25.792 9 X,R=0.999 999。【结论】紫外分光光度法和高效液相色谱法测定萘普生注射液中萘普生含量的结果差异较小,建立的高效液相色谱法可以对萘普生注射液进行快速检测。【Objective】Comparison of HPLC and UV-VIS spectrophotometer for the detection of Naproxen in Naproxen Injection.【Objective】 The aim of this paper is to compare the content of naproxen in Naproxen Injection detected by UV spectrophotometry and high performance liquid chromatography（HPLC）.【Methods】Naproxencontent was determined by HPLC （ velocity： 1. 0 mL /min; wavelength, 272nm） and UV spectrophotometer（ wavelength： 331 nm ） respectively. The contrast samples were Sodium hydroxide for UV spectrophotometry. 【Results】 The mean recovery of HPLC analysis was 99.08%; RSD was 0.5%, less than standard values 2%;Linear equation is Y = 4.326 9 ＋ 25.792 9 X , R =0.999 999. 【Conclusion】 The results of UV spectrophotometry and HPLC have litte difference,which suggests that it can be used for the rapid inspection methods of Naproxen Injection by established HPLC instead of by UV spectrophotometry.

关 键 词：萘普生 含量测定 高效液相色谱法 紫外分光光度法 

分 类 号：S851.4[农业科学—预防兽医学]