不同采收情况与干蟾皮中酯类成分含量对比研究  

The Comparative Study of the Contents of Ester Compounds in Bufo Bufo Gargarizans from Different Product

作　　者：周谧 傅兴圣 ZHOU Mi;FU Xing-sheng(Nantong Food and Drug Control Center,Nantong226006,China)

机构地区：[1]江苏省南通市食品药品监督检验中心,南通226006

出　　处：《海峡药学》2018年第10期41-44,共4页Strait Pharmaceutical Journal

摘　　要：目的对不同采收情况与干蟾皮中华蟾酥毒基和脂蟾毒配基含量对比研究,为其药材质量评价与资源合理利用提供参考。方法建立高效液相色谱(HPLC)法对不同采收情况的干蟾皮中华蟾酥毒基和脂蟾毒配基进行测定。色谱柱:迪马C_(18)(5μm,4. 6×250mm);流动相:乙腈-0. 5%磷酸二氢钾(50∶50)(用磷酸调节pH值3. 2);流速:1. 0mL·min^(-1),柱温:30℃,检测波长:296nm。结果测定的9批干蟾皮中,不同采收情况的干蟾皮含量的差异较大。测定华蟾酥毒基和脂蟾毒配基的色谱分离良好,浓度和峰面积呈良好的线性关系,线性范围分别为1. 02~50. 88μg·mL^(-1),r=0. 9999(n=6); 0. 97~48. 54μg·mL^(-1),r=0. 9999(n=6)。平均加样回收率分别为99. 97%(n=9,RSD=2. 87%)、97. 74%(n=9,RSD=2. 03%)。结论干蟾皮合理的采收方法是取耳后腺未刮去浆的中华大蟾蜍剥取外皮,除净肌肉纤维,立即贴于板上或撑开,晾干。OBJECTIVE To make a contrastive study about comparing the contents of cinobufagin and resibufogenin in Bufo bufo gargarizans from different product,and provide information for its medical material quality appraise and thereasonable utilization of resources. METHODS HPLC was established to determina the contents of cinobufagin and resibufogenin in Bufo bufo gargarizans of different product.The DIKMA C 18 （250mm×4.6mm,5μm）was used.The mobile phase was the sytem of acetonitrile-0.5% dihydrogen phosphate（50：50）（use phosphoric acid to adjust pH value to 3.2）.The flow rate was 1.0mL·min-1 .The column temperature was 30℃ and the detection wavelength was 296nm. RESULTS In the detected 9 batch samples,while there was a certain difference in Bufo bufo gargarizans of different product.The linear rangeof cinobufagin was 1.02～50.88μg·mL -1 ,r=0.9999（n=6）,the average recovery was 99.97%（n=9,RSD=2.87%）.The linear range of resibufogenin was 0.97～48.54μg·mL -1 ,r=0.9999（n=6）,the average recovery was 97.74%（n=9,RSD=2.03%）. CONCLUSION The reasonable method of harvesting Bufobufo gargarizans is to peel off the skin of the Bufo bufo gargarizans with parotid gland remained and muscle fibre detached,immediately pressed against the board and dry out.

关 键 词：干蟾皮 采收情况 华蟾酥毒基 脂蟾毒配基 

分 类 号：R282[医药卫生—中药学]