miR-3077-5P对3T3-L1前脂肪细胞成脂分化能力的影响  

Effects of miR-3077-5P on adipogenic differentiation of 3T3-L1 preadipocyte

作　　者：杨晓红[1] 杨琨[2] 廖立 金岩 Yang Xiaohong;Yang Kun;Liao Li;Jin Yan(Department of Prosthodontic,Affiliated Stomatological Hospital of Zunyi Medical University,Zunyi Guizhou 563099,China;Department of Periodontal,Affiliated Stomatological Hospital of Zunyi Medical University,Zunyi Guizhou 563099,China;Research and Development Center for Tissue Engineering,Hospital of Stomatology,Fourth Military Medical University,Xi＇an Shanxi 710032,China)

机构地区：[1]遵义医学院附属口腔医院口腔修复科,贵州遵义563099 [2]遵义医学院附属口腔医院牙周科,贵州遵义563099 [3]第四军医大学口腔医院组织工程研究中心,陕西西安710032

出　　处：《遵义医学院学报》2018年第5期584-588,共5页Journal of Zunyi Medical University

基　　金：贵州省科技厅联合基金资助项目(NO:黔科合LH字[2015]7534)

摘　　要：目的观察miR-3077-5P对3T3-L1前脂肪细胞成脂分化能力的影响。方法通过细胞转染技术分别上调和下调3T3-L1前脂肪细胞中的miR-3077-5P表达,将其分为对照(control)组、模拟物(mimics)组及抑制物(inhibitor)组;将各组3T3-L1前脂肪细胞成脂诱导10 d,以逆转录聚合酶链式反应(Reverse transcription-polymerase chain reaction,RT-PCR)法检测其相关成脂基因PPAR-γ和LPL mRNA表达水平,用免疫蛋白印迹(Western Blot,WB)法检测其相关成脂基因PPAR-γ和FABP4蛋白水平;采用油红O染色观察脂滴形成情况并作定量分析。结果成脂诱导10 d,miR-3077-5P mimics组成脂基因PPAR-γ和LPL mRNA的表达水平及PPAR-γ和FABP4蛋白水平均升高,而miR-3077-5P inhibitor组则相反(P均<0. 05);成脂诱导10 d,miR-3077-5P mimics组脂滴形成最大,脂滴形成量存在升高现象,miR-3077-5P inhibitor组脂滴较小且形成量较少(P均<0. 05)。结论 miR-3077-5P对3T3-L1前脂肪细胞成脂分化过程具有促进作用。Objective To investigate the effects of miR-3077-5P on 3T3-L1 adipogenic differentiation. Methods miR-3077-5P mimics,inhibitors and negative control were transfected into 3T3-L1 cells.The mRNA expressions of PPAR-γ and LPL were detected by real time RT-PCR and protein level of PPAR-γ and FABP4 were detected by Western Blot assay after adipogenic induction for 10 days.Oil-red staining was performed and quantified in 3T3-L1 cells. Results After adipogenic induction for 10 days,Overexpression of miR-3077-5P by the mimics,the mRNA expression of PPAR-γ and LPL and protein expression level of PPAR-γ and FABP4 were increased in miR-3077-5P mimics treatment group,whereas these genes expressions were decreased in miR-3077-5P inhibitors treatment group.Moreover,Oil-red staining showed that more formation of lipid droplets was shown in miR-3077-5P mimics treatment group than that in miR-3077-5P inhibitors treatment group. Conclusion miR-3077-5P could promote the adipogenic differentiation of 3T3-L1 cells.

关 键 词：成脂分化 miR-3077-5P 3T3-L1前脂肪细胞 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]