肠愈宁对溃疡性结肠炎大鼠炎性因子及钙卫蛋白释放的影响  

Effects of Changyuning on Inflammatory Factors and the Release of Calprotectin in Rats with Ulcerative Colitis

作　　者：王海强[1] 郑丽红[2] 朱峰[3] 刘朝霞[1] 王静滨[1] 张杨[1] 梁国英[1] 刘金狄 张月涛 Wang Haiqiang;Zheng Lihong;Zhu Feng;Liu Zhaoxia;Wang Jingbin;Zhang Yang;Liang Guoying;Liu Jindi;Zhang Yuetao(The First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China;The SecondAffiliated Hospital of Heilongjiang University of Traditional Chinese Medicine,Harbin 150001,China;HeilongjiangUniversity of Traditional Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学附属第一医院,哈尔滨150040 [2]黑龙江中医药大学附属第二医院,哈尔滨150001 [3]黑龙江中医药大学,哈尔滨150040

出　　处：《世界中医药》2018年第10期2547-2551,共5页World Chinese Medicine

基　　金：黑龙江省自然科学基金项目(H2016077;H2017061);国家自然科学基金项目(81273685);黑龙江中医药大学科研基金(201304);全国名老中医谢晶日传承工作室

摘　　要：目的:研究肠愈宁颗粒对二硝基氯苯乙酸复合法诱导大鼠溃疡性结肠炎的作用,并初步探讨其作用机制。方法:将雄性SD大鼠分成6组:正常组、模型组、柳氮磺胺吡啶组(SASP)和肠愈宁颗粒低、中、高剂量组。以二硝基氯苯乙酸复合法诱导大鼠溃疡性结肠炎模型。评估疾病活动指数(DAI)、结肠黏膜损伤指数(CMDI)和病理组织学。ELISA方法测定血清IL-β、TNF-α、钙卫蛋白(Calproteein,CP),Western blot方法检测结肠黏膜CP的表达水平。结果:DAI结果提示造模成功,与正常组比较,模型组血清中IL-β、TNF-α、CP均明显升高,而CMDI指数、结肠黏膜CP均明显升高。肠愈宁颗粒各组DAI、CMDI、结肠组织中黏膜CP均降低,血清中IL-β、TNF-α、CP水平也明显降低。结论:肠愈宁颗粒能够有效缓解二硝基氯苯乙酸复合法诱导的大鼠溃疡性结肠炎,其作用机制可能与下调IL-β、TNF-α表达,减少CP水平有关。Objective To study the effects of Changyuning Granule （CYN） on ulcerative colitis induced by dinitrochlorobenzene/ethanoic acid in rats,and to explore its mechanism. Methods： Male SD rats were divided into 6 groups：the normal group,the model group,the low,medium and high dose group of CYN and the sulfonylamine group （SASP）.The ulcerative colitis model was induced by dinitrochlorobenzene/ethanoic acid.Disease activity index （DAI）,colonic mucosa damage index （CMDI） and pathological histology were assessed.Serum IL-β,TNF-α,and calcium protein （Calproteein,CP） were detected by ELISA.The expression of CP in colonic mucosa was detected by Western blot. Results： DAI results suggested that the model was successful.Compared with the normal group,IL-β,TNF-α,and CP in the serum of the model group were significantly increased,and the index of CMDI,colonic mucosa CP were significantly increased.The DAI,CMDI,and mucosal CP in the colon tissue of each group of Changyuning Granules were decreased,and the levels of IL-,TNF-,and CP in serum were also significantly decreased. Conclusion： CYN Granule can effectively relieve the ulcerative colitis induced by dinitrochlorobenzene/ethanoic acid in rats.The mechanism may be related to down-regulation of IL-β and TNF-α expression and reduction of CP level.

关 键 词：肠愈宁 溃疡性结肠炎 IL-Β TNF-Α CP 

分 类 号：R285.5[医药卫生—中药学]