内毒素经不同给药途径建立新生鼠急性肺损伤模型对比分析  

Compared with different animal models of lung injury in neonate rats induced by different doges and ways of lipopolysaccharides

作　　者：罗艳[1] 李清香 曹云涛[1] LUO Yan;LI Qingxiang;CAO Yuntao(Department of Neonatology,AJfiliated Hospital of Zunyi Medical College,Zunyi,Guizhou 563003,China)

机构地区：[1]遵义医学院附属医院新生儿科,贵州遵义563003

出　　处：《现代医药卫生》2018年第21期3290-3294,共5页Journal of Modern Medicine & Health

基　　金：遵义医学院资助项目(院字2012(02)号)

摘　　要：目的对比分析内毒素(LPS)经不同途径进入新生鼠体内对建立急性肺损伤模型的影响。方法将新生清洁级SD大鼠分为三组:鼻腔滴药组[包括对照组和LPS组,LPS组给予鼻腔中滴入不同剂量LPS(3、4、5、6、8 mg/kg),对照组给予鼻腔滴入等量生理盐水]、气管给药组[包括对照组和LPS组,LPS组给予气管中滴入不同剂量LPS(LPS量同鼻腔滴药组),对照组给予气管滴入等量生理盐水]、腹腔给药组[包括对照组和LPS组,LPS组给予腹腔注入不同剂量LPS(LPS量同鼻腔滴药组),对照组给予腹腔注入等量生理盐水]。然后分别在1、6 h统计新生鼠的死亡率,并依据上述实验结果选定死亡率较低的LPS剂量。新生鼠给予选定的LPS及等量生理盐水后记录各组新生鼠肺的病理评分、湿/干重比值(W/D)及支气管肺泡灌洗液(BALF)中多形核白细胞(PMN)计数。结果 (1)不同途径的LPS组中随着药物剂量递减,新生鼠的死亡率逐渐降低,当LPS剂量小于5 mg/dL时新生鼠死亡率可降低至25%以下。(2)当LPS剂量为3 mg/dL时,鼻腔滴药组、气管给药组、腹腔给药组各个时间点的炎症病理评分与对照组比较,差异均无统计学意义(P>0.05);但时间点为6、12 h时W/D值、BALF中PMN值均显著高于对照组,其中时间为24 h时PMN值仍高于对照组,差异均有统计学意义(P<0.05)。当LPS剂量为4 mg/dL或5 mg/dL,时间点为给予LPS后6、12 h时,炎症病理评分及W/D值、BALF中PMN值均显著高于对照组,其中时间为24 h时PMN值仍高于对照组,其他时间点PMN值比较,差异无统计学意义(P>0.05)。(3)不同给药途径的对照组肺泡灌洗液中PMN比较发现,气管给药组中PMN值显著增加。结论 LPS的3种不同给药途径均可制备新生鼠急性肺损伤模型。但因气管给药为有创操作,可能导致新生鼠感染,故不推荐选取该途径给药建立肺损伤模型进行实验研究。Objective To explore the different influence among different animal models of lung injury in neonate rat induced by different doges and ways of lipopolysaccharides （LPS）. Methods Neonatal SD rats were divided into three groups ： intranasal instillation groups （control group and LPS group）, intratracheal administration groups （control group and LPS group） and intraperitoneal administration groups （control group and LPS group）. Intranasal instillation groups were divided into the control group and the LPS experimental group. In the LPS groups, 3,4,5,6 and 8 mg/kg LPS were dropped into the nasal cavity of rats respectively. In the control group, equal amount of saline were dropped into the nasal cavity- of rats. Intratracheal administration groups were divided into the control group and the LPS experimental group.In the LPS groups, 3,4,5,6 and 8 mg/kg LPS were dropped into the trachea respectively. In the control group, equal amount of saline were dropped into the trachea. In- traperitoneal administration group were also divided into the control group and the LPS experimental group.Rats were injected intraperitoneally with 3,4,5,6 and 8 mg/kg LPS, respectively-, in the LPS groups and with equal amount of saline in the control group.Then the mortality ratio of rats were measured at 1,6 h after given the LPS. According to the statistical results ,we next se- lected low mortality doses of LPS （3,4 and 5 mg/kg）.Score inflammatory characteristics of lung sections, wet/dry weight ratio （W/D） of lung lobes and cell（PMN） count in bronchoalveolar lavage fluid （BALF） were detected at 1,6,12,24,72 h after giv- en the LPS, respectively. Results （1） The mortality ratio of rats were decreased gradually with the decreasing LPS doses. When the dosages of LPS was less than 5 mg/kg. （2）When the dosages of LPS was 3 mg/kg, inflammatory characteristics of lung sections at various hours that were no differences among intranasal instillation groups, intratracheal administration groups and intrape

关 键 词：呼吸窘迫综合征 脂多糖类 大鼠 Sprague-Dawley 婴儿 新生 

分 类 号：R722.1[医药卫生—儿科] R-332[医药卫生—临床医学]