樱花品种‘染井吉野’启动培养初步研究  

A Preliminary Study on the Seed Cultivation of Prunus yedoensis

作　　者：夏明霞[1] 张宁宁[1] 江兴瑜 张琼[1] 衡燕[1] XIA Ming-xia;ZHANG Ning-ning;JIANG Xing-yu;ZHANG Qiong;HENG Yan(Nanjing Institute of Agricultural Science in Jiangsu Hilly Region,Nanjing 210046,China)

机构地区：[1]江苏丘陵地区南京农业科学研究所,江苏南京210046

出　　处：《金陵科技学院学报》2018年第3期78-80,共3页Journal of Jinling Institute of Technology

基　　金：江苏省林业三新工程项目(LYSX[2015]29)

摘　　要：以春季萌发的樱花‘染井吉野’带腋芽嫩茎段为试材,进行了外植体启动培养研究。结果表明:最适外植体为樱花当年生未木质化嫩枝的中部;最佳消毒时间为先用70%的酒精消毒30s再用0.1%的HgCl2消毒10min;适宜的启动培养基为WPM+6-BA 1.5mg·L-1+NAA 0.1mg·L-1,诱导率达66.67%。The starting culture of explant was carried out with tender stem section of Prunus yedoensis with axillary bud,which was germinated in spring.The experimental results showed that the most suitable explant was the middle part of the cherry wood;the best disinfection time is to disinfect 30 sfirst with 70% alcohol and then disinfect 10 min by 0.1% of HgCl2;the optimal starting medium was WPM＋6-BA 1.5 mg·L-1＋NAA 0.1 mg·L-1 and the induction rate was 66.67%.

关 键 词：樱花 外植体 启动培养 

分 类 号：S685.99[农业科学—观赏园艺]