用正交试验法优化香水百合试管鳞茎的诱导  

Optimization of Test-tube Bulblet Inducement of Lilium Oriental Hybrid ‘Casa Blanca'through Orthogonal Test

作　　者：祁宏英[1,2] 徐洪国 杨伟丽[1] QI Hong-ying;XU Hong-guo;YANG Wei-li(College of Life Science,Agriculture and Forestry,Qiqihaer University,Qiqihaer 161006,China;Heilongjiang Provincial Key Laboratory of Resistant Gene Engineering and Protection of Biodiversity in Cold Area,Qiqihaer 161006,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006 [2]抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,黑龙江齐齐哈尔161006

出　　处：《江西农业学报》2018年第11期32-34,共3页Acta Agriculturae Jiangxi

基　　金：黑龙江省自然科学基金项目(C2017.67);黑龙江省教育厅基本业务专项(135209263)

摘　　要：通过正交试验研究了不同浓度(0～0.5 mg/L) NAA、不同浓度(0.5～2.0 mg/L) 6-BA、不同鳞片层次(外层、中层、内层)及不同鳞片部位(上部、中部、基部)对香水百合试管鳞茎诱导的影响,并优化了诱导试管鳞茎的组织培养条件。结果表明:各因素对诱导试管鳞茎的影响大小为NAA>6-BA>鳞片层次>鳞片部位;试管鳞茎诱导的最佳组合条件是MS基本培养基+0.1 mg/L NAA+2.0 mg/L 6-BA+中层鳞片+鳞片中部部位,在此条件下诱导香水百合试管鳞茎,诱导率达到100%,平均分化数达到4.89。Orthogonal tests were carried out to study the influences of different concentrations （0-0.5 mg/L） of NAA, different concentrations （0.5-2.0 mg/L） of 6-BA, different scale layers （outer layer, middle layer and inner layer） and different scale positions （top, middle part and base） on the test-tube bulblet inducement from scales of Lilium oriental hybrid ‘Casa Blanca’, and the tissue culture conditions for the inducement of test-tube bulblet were optimized. The results indicated that the influences of various factors on the inducement of test-tube bulblet exhibited the following order： NAA〉6-BA〉scale layer〉scale position. The optimum combined conditions for the inducement of test-tube bulblet were MS basic culture medium＋ 0.1 mg/L NAA＋ 2.0 mg/L 6-BA＋ middle-layer scale＋ middle-part scale. Under these best conditions, the inducement rate of test-tube bulblet from scales of Lilium oriental hybrid ‘Casa Blanca’ reached 100%, and the average differentiation number reached 4.89.

关 键 词：香水百合 鳞茎 诱导 组织培养 正交设计 优化 

分 类 号：S682.29[农业科学—观赏园艺]