HPLC法测定蒙药哈敦嘎日迪-13丸中木香烃内酯和去氢木香内酯的含量  

To determinate the contents of Costunolide and Dehydrocostus lactone in Mongolian Medicine Hadungaridi-13 Pill by HPLC

作　　者：安明[1] 薄彧坤[1] 杨丹[1] 张慧敏 邬国栋[1] An Ming;Bo Yu Kun;Yang Dan;ZhangHuimin;Wu Guo Dong(Baotou Medical College,Baotou 014060,China)

机构地区：[1]包头医学院,内蒙古包头014060

出　　处：《中国民族医药杂志》2018年第10期45-49,共5页Journal of Medicine and Pharmacy of Chinese Minorities

基　　金：内蒙古自治区高等学校科学研究项目(NJZY18197);包头医学院“花蕾计划”研究项目(NJZY18197)

摘　　要：目的:建立高效液相色谱法测定哈敦嘎日迪-13丸中木香烃内酯和去氢木香内酯含量的方法。方法:采用高效液相色谱法同时测定处方中的木香烃内酯和去氢木香内酯含量,色谱条件:C18色谱柱(150mm×4. 6mm,5μm);柱温:30℃,流动相:甲醇-水(65:35),检测波长:225nm,流速:1. 0mL/min。结果:色谱峰分离情况良好,木香烃内酯在0. 2148~1.202888μg·m L-1范围内与峰面积呈良好的线性关系(r=0. 99998)。去氢木香内酯在0. 6347~1. 6926μg·m L-1的范围内与峰面积呈现良好的线性关系(r=0. 99995)。木香烃内酯平均加样回收率为(n=9)为102. 24%(RSD=0. 96%),去氢木香内酯平均加样回收率为(n=9)为104. 46%(RSD=1. 99%)。结论:本法所建立的含量测定方法简便准确、专属性强、重复性好,适用于哈敦嘎日迪-13丸的质量控制。Objective To determinate the contents of Costunolide and Dehydrocostus lactone in Hadun Garidi-13 pills. Methods An HPLC method was used and C 18 was selected（150×4.6mm,5μm）, acolumn temperature was 30℃, methyl alcohol-water（65：35） were mobile phase the detecting wave length and velocity were 225 nm and 1.0 mL/min repectively. Results There were established a good linear relation between 0.2148～1.202888μg·mL -1 of Costunolide, r=0.99998, 0.6347～1.6926μg·mL -1 of Dehydrocostus lactone , r=0.99995, Costunolide the average recovery was 102.24% , RSD was 0.96%, Dehydrocostus lactone the average recovery was 104.46% , RSD was 1.99%. Conclusion The method had the advantages of simple and easy operation, sensitive and accurate and exhibited a good separation effect. The methods can be used to the determination of contents and quality control of the Hadun Garidi-13 pills.

关 键 词：高效液相色谱法 蒙药 哈敦嘎日迪-13丸 木香烃内酯 去氢木香内酯 

分 类 号：R291.2[医药卫生—民族医学]