时间分辨荧光免疫法检测环境中四溴双酚A衍生物(TBBPA DHEE)  

A sensitive time-resolved fluoroimmunoassay for the detertion of tetrabromobisphenol A bis (2-hydroxyetyl) ether in environments

作　　者：黄梦璐 张祯 吴向阳 HUANG Menglu ZHANG Zhen WU Xiangyang(School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China)

机构地区：[1]江苏大学环境与安全工程学院,镇江212013

出　　处：《环境化学》2018年第10期2124-2130,共7页Environmental Chemistry

基　　金：国家自然科学基金(21577051)资助~~

摘　　要：本研究利用前期制备的四溴双酚A双(2-羟基乙基)醚(TBBPA DHEE)的多克隆抗体,构建了一种高灵敏的时间分辨荧光免疫分析法,用于检测环境中的四溴双酚A衍生物TBBPA DHEE.为提高分析方法的灵敏度,本研究对反应体系中有机溶剂(甲醇)、离子强度及缓冲体系p H等参数进行了优化.在最适条件下,本方法的最低检测限(LOD,基于F/F_0=90%)与IC_(50)分别为0.27 ng·m L^(-1)及4.3 ng·m L^(-1).该方法具有较高的准确度(水样加标回收率为96%—120%;土样及生物加标回收率为75%—90%).利用该方法对江苏省苏州某地区的环境样品进行了调查:在采集的56个样本中,13个样本检出了这种污染物.检出浓度为:水样0.5—2.7 ng·m L^(-1)、土样0.6—1.6 ng·g^(-1),干重、生物样2.9—4.6 ng·g^(-1),湿重.本研究为首次针对环境中TBBPA DHEE污染状况的系统性分析.A sensitive time-resolved fluoroimmunoassay（TRFIA）was developed using our previously produced polyclonal antibody for the determination of tetrabromobisphenol A bis（2-hydroxyetyl）ether（TBBPA DHEE）,one of typical tetrabromobisphenol A（TBBPA）derivatives.The established method showed satisfactory sensitivity under the optimized conditions.The detection limit（LOD,based on 90%F/F_0）and IC_（50）calculated from the standard curve were0.27 and 4.3 ng·m L^（-1）for TBBPA DHEE,respectively.Using the TRFIA method,a systematic investigation was implemented at a halogenated flame retardants plant（main sampling site）in Suzhou,Jiangsu province,China.As a result,TBBPA DHEE was found in 5 of the 26 water samples with concentrations of 0.5—2.7 ng·m L^（-1）,5 of 20 soil samples with concentrations of 0.6—1.6 ng·g^（-1）（dry weight,dw）,and 3 out of 10 fist samples with concentrations of 2.9—4.6 ng·g^（-1）（wet weight,ww）.To our knowledge,this is the systematic investigation of TBBPA DHEE about its occurrence in the environment.

关 键 词：时间分辨荧光免疫法 多克隆抗体 四溴双酚A衍生物 四溴双酚A双(2-羟基乙基)醚 环境污染调查 

分 类 号：X832[环境科学与工程—环境工程]