益母草碱对肥大心肌细胞p38MAPK及下游基因表达的影响研究  

Effects of Leonurine on p38MAPK and Downstream Gene-expressionin Cardiomyocyte Hypertrophy

作　　者：卢丽娜[1] 袁露[1] 梁赵文 罗建华[3] 杨辉[1] 李利[1] 杨冬花 LU Lina;YUAN Lu;HANG Zhaowen;LUO Jianhua;YANG Hui;LI Li;YANG Donghua(Department of Medicine for Cadre,The People,s Hospital of Guizhou Province,Guiyang 550002,Guizhou,China;Guiyang TCM College,Guiyang 550002,Guizhou,China;Department of Endocrinology,The People,s Hospital of Guizhou,Guiyang 550002,Guizhou,China)

机构地区：[1]贵州省人民医院干部医疗科,贵州贵阳550002 [2]贵阳中医学院,贵州贵阳550002 [3]贵州省人民医院内分泌科,贵州贵阳550002

出　　处：《中华中医药学刊》2018年第11期2614-2617,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81560735)

摘　　要：目的：探讨益母草碱对肥大心肌细胞p38MAPK及下游基因表达的影响。方法：以血管紧张素Ⅱ（AngⅡ）诱导肥大心肌细胞,在此基础上给予p38丝裂原活化蛋白激酶（p38MAPK）抑制剂（SB203580,10μmol/L）及低、中、高浓度益母草碱（5、10、20μmol/L）干预。分别以[~3H]亮氨酸掺入量、酶联免疫吸附测定（ELISA）和硝酸还原酶法检测心肌细胞蛋白质合成速率以及细胞上清B型利钠肽（BNP）、内皮素-1（ET-1）、AngII和一氧化氮（NO）含量;Real-time PCR法检测心肌细胞ET-1、p38MAPK、肌细胞增强因子2（MEF2）、β-肌球蛋白重链（β-MHC）、α-肌球蛋白重链（α-MHC） mRNA的表达水平。结果：与模型组比较,高剂量益母草碱组心肌细胞蛋白质合成速率、BNP、ET-1及AngII含量明显降低（P〈0.05）,同时ET-1、p38MAPK、 MEF2、β-MHC mRNA表达水平明显下调（P〈0.05）,而NO含量和α-MHC mRNA表达水平明显上调（P〈0.05）。结论：高剂量益母草碱（20μmol/L）可能通过调节p38MAPK及下游基因表达逆转心肌细胞肥大。Objective： To investigate the effects of Leonurine on p38 MAPK and downstream gene-expression in cardiomyocyte hypertrophy. Methods： The cardiomyocyte hypertrophy was induced by AngⅡ（0.1 μmol/L）in primary neonatal cardiomyocytes. The cells were treated with p38 MAPK inhibitor（SB203580, 10 μmol/L） and different concentrations of Leonurine（5 μmol/L,10 μmol/L, 20 μmol/L）.The protein synthesis was assayed with [~3H]-leucine incorporation.The contents of BNP,ET-1 and AngII were detected by ELISA,and that of NO by Nitrate reductase. The mRNA expressions of ET-1,p38 MAPK,MEF2,β-MHC and α-MHC were detected by Real-time PCR.Results： The protein synthesis,the contents of BNP,ET-1 and AngII, and the mRNA expressions of ET-1, p38 MAPK,MEF2 and β-MHC were lower,but the contents of NO and the mRNA expression of α-MHC in high-dose Leonurine group were higherthan those in model group（all P〈0.05）.Conclusion：Leonurine（20 μmol/L） could inhibit cardiomyocyte hypertrophy induced by AngⅡ, and the mechanism could be related to there gulating the p38 MAPK and downstream gene-expression.

关 键 词：益母草碱 心肌细胞肥大 P38丝裂原活化蛋白激酶 肌细胞增强因子2 

分 类 号：R286[医药卫生—中药学]