小双节RNA病毒阳性腹泻患儿粪便标本的病毒宏基因组学分析  

Viral metagenomic analysis of fecal samples from diarrhea children with positive picobirnaviruses

作　　者：杨凤真 伊茂礼[1] 吴金英[1] 梁英凤[1] 臧琴波[1] 姜丽华[1] 周志美 孙光明 Yang Fengzhen;Yi Maoli;Wu Jinying;Liang Yingfeng;Zang Qinbo;Jiang Lihua;Zhou Zhimei;Sun Guangming(Medical Laboratory,Yantai Yuhuangding Hospital,Yantai 264000,Shangdong Province,Chin;Medical Laboratory,Xuzhou Central Hospital,Xuzhou 221000,Jiangsu Province,China)

机构地区：[1]烟台毓璜顶医院检验科,264000 [2]徐州市中心医院检验科,221000

出　　处：《中华实用儿科临床杂志》2018年第19期1495-1498,共4页Chinese Journal of Applied Clinical Pediatrics

摘　　要：目的研究小双节RNA病毒（PBVs）与儿童不明原因腹泻的关系。方法2015年1－12月于徐州市中心医院临床微生物检验中心为采样地点，筛选PBV反转录-PCR阳性的6岁以下腹泻患儿粪便样本作为研究组，同时从幼儿园采集4例正常儿童粪便样本作为对照组。利用病毒宏基因组学方法对上述样本进行分析，探讨PBVs与儿童腹泻的关系。对分离的PBV序列中RdRp行进化分析，进一步探讨PBVs分型与儿童腹泻的关系。结果7例腹泻儿童粪便样本含有高浓度的PBV序列，而阴性对照组只有1例含有低浓度的PBV序列，其他3例不含PBV序列。对分离到的PVB序列中RdRp进行分析，7个RdRp序列分别来源于7例粪便标本，分别命名为ChXz-1至ChXz-7。根据预测的RdRp的氨基酸序列与GenBank中已知的RdRp序列进行进化分析，提示7例PVB序列从属于3个不同的基因型，ChXz-1、ChXz-2、ChXz-3从属于基因型Ⅰ，ChXz-4、ChXz-5、ChXz-7属于基因型Ⅱ，ChXz-6属于基因型Ⅲ。结论PVBs可能是导致儿童腹泻的病原体之一，且3型PVBs均有可能导致腹泻的发生。ObjectiveTo investigate positive picobirnaviruses（PVBs） infection and its association with unexplained diarrhea in children.MethodsFrom January to December 2015, the Clinical Microbiology Testing Center of Xuzhou Central Hospital as the sampling location, 7 PVB reverse transcription-polymerase chain reaction（RT-PCR）-positive fecal samples with diarrhea were collected from children under 6 years old and 4 samples from healthy children were obtained and all the samples were analyzed by viral metagenomics to investigate the relationship between PVBs and diarrhea in children.Phylogenetic analysis of RdRp in the isolated PVB sequences was carried out to clarify the relationship between PVB classification and diarrhea.ResultsAll the 7 diarrhea feces contained high titers of PVB sequences, while 3 of the controls were negative, and 1 with low titers of PVB.RdRp analysis was carried out on the isolated PVB sequences, which displayed that 7 RdRp sequences caming from 7 fecal samples separately, so named ChXz -1 to ChXz -7 respectively.Phylogenetic analysis based on the predicted amino acid sequences of RdRp from this study and whole RdRp sequences available in the GenBank database indicated that the 7 RdRp sequences belonged to 3 genogroups, in which ChXz-1, ChXz-2, ChXz-3 belonging to genogroup Ⅰ, ChXz-4, ChXz-5, ChXz-7 belonging to genogroup Ⅱ, and ChXz-6 belonging to genogroup Ⅲ.ConclusionsPVBs might be the cause of diarrhea in children in this study, and all the 3 different PVBs may lead to diarrhea.

关 键 词：小双节RNA病毒 腹泻 病毒宏基因组学 

分 类 号：R725.7[医药卫生—儿科]