miR-31在亚洲玉米螟中的表达及其与Cry1Ab蛋白敏感性关系研究  

Expression of miR-31 in Asian Corn Borer(Ostrinia furnacalis) and Its Relationship with Cry1Ab Protein Sensitivity

作　　者：徐丽娜 周子燕 胡飞 何康来[2] 胡本进 XU Li-Na;ZHOU Zi-Yan;HU Fei;HE Kang-Lai;HU Ben-Jin(Institute of Plant Protection and Agro-Products Safety,Anhui Academy of Agricultural Sciences,Hefei 230031,China;Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]安徽省农业科学院植物保护与农产品质量安全研究所,合肥230031 [2]中国农业科学院植物保护研究所,北京100193

出　　处：《农业生物技术学报》2018年第11期1962-1969,共8页Journal of Agricultural Biotechnology

基　　金：植物病虫害生物学国家重点实验室开放基金(No.SKLOF201704)

摘　　要：昆虫体内苏云金杆菌(Bacillus thuringiensis, Bt)受体蛋白表达量的改变是影响靶标昆虫对Bt产生抗性的关键因素之一,但是其表达调控机制并不清楚。微小RNA (microRNA, miRNA)是一类非编码RNA,可与靶标mRNA互补配对,在转录后水平调控基因表达,进而参与昆虫生长、发育和逆境应答等多种生物学功能的调控。为了探讨microRNA-31 (miR-31)在亚洲玉米螟(Asian corn borer, ACB)(Ostrinia furnacalis)对杀虫晶体蛋白(pesticidal crystal protein, Cry1Ab)产生抗性过程中的作用,本研究分析了miR-31在亚洲玉米螟Cry1Ab抗性品系(ACB-AbR)和Bt敏感品系(ACB-BtS)不同组织的表达,结果显示,miR-31在ACB-AbR表皮组织中的表达量显著高于ACB-BtS表皮组织,而在ACB-BtS和ACB-AbR中肠的表达量却显著下降,而且miR-31在ACB-AbR中肠的表达量也低于其在ACB-BtS中的表达量。进而以Sf9细胞为研究对象,分析miR-31表达量改变后,细胞对Cry1Ab杀虫蛋白敏感性的变化。研究发现miR-31表达量升高导致Sf9对Cry1Ab杀虫蛋白敏感性增加;反之,miR-31表达量降低导致Sf9对Cry1Ab杀虫蛋白敏感性减弱。上述结果提示,亚洲玉米螟Cry1Ab抗性品系幼虫中肠miR-31表达量下降,可能是导致该虫对Cry1Ab杀虫蛋白产生抗性的原因之一。本研究为进一步解析亚洲玉米螟Bt抗性产生的机理提供了参考依据。The change of Bacillus thuringiensis （Bt） receptor protein expression is one of the key factors affecting the resistance of target insects to Bt, but the mechanism of its expression regulation is not clear. MicroRNAs （miRNAs）, as a class of non-coding RNAs, could be paired with target mRNA to regulate gene expression at post-transcriptional level, and thus participate in the regulation of insect growth, development and stress response. To investigate the role of microRNA-31 （miR-31） in the production of resistance of Asian corn borer （ACB, Ostrinia furnacalis） to pesticidal crystal protein （Cry1Ab）, the expression of miR-31 in different tissues of Cry1Ab resistant strain of ACB （ACB-AbR） and Bt sensitive strain （ACB-BtS） was analyzed. It was found that the expression of miR-31 in the epidermis of ACB-AbR was significantly higher than that in the epidermis of ACB-BtS, but decreased significantly in the midgut of ACB-BtS and ACB-AbR, and the expression of miR-31 in the midgut of ACB-AbR was lower than that in the midgut of ACB-AbR. Then, the sensitivity of Sf9 cells to Cry1Ab was analyzed after the expression of miR-31 was changed. It was found that the increased expression of miR-31 resulted in an increase of the sensitivity of Sf9 to Cry1Ab, whereas the decrease in the expression of miR-31 resulted in a decrease of the sensitivity of Sf9 to Cry1Ab. These results suggested that the decrease of miR-31 expression in midgut of ACB-AbR may be one of the reasons leading to its resistance to Cry1Ab. This study provides basic data for further analysis of the mechanism of Bt resistance in ACB.

关 键 词：miR-31 亚洲玉米螟(ACB) 杀虫晶体蛋白(Cry1Ab)抗性 

分 类 号：S435.132[农业科学—农业昆虫与害虫防治]