延伸因子EF-Tu在鼠伤寒沙门菌细胞表面的定位分析  

Identification of cell surface localization of elongation factor Tu in Salmonella typhimurium

作　　者：明淑贞 张怡聪[1,2,3] 孙娜 赵宁宁 赵孝民 王方昆[1,2,3] MING Shu-zhen;ZHANG Yi-cong;SUN Na;ZHAO Ning-ning;ZHAO Xiao-min;WANG Fang-kun(College of Animal Science and Technology,Shandong A gricultural University,Tai＇an 271018,China;Shandong Provincial Key Laboratory of Animal Biotechnalogy and Disease Control and Prevention,Tai＇an 271018,China;Shandong Provincial Engineering Technology Research Center of A nimal Disease Control and Prevention,Tai＇an 271018,China)

机构地区：[1]山东农业大学动物科技学院,山东泰安271018 [2]山东省动物生物工程与疾病防治重点实验室,山东泰安271018 [3]山东省畜禽疫病防制工程技术研究中心,山东泰安271018

出　　处：《中国兽医科学》2018年第11期1381-1387,共7页Chinese Veterinary Science

基　　金：国家自然科学基金青年基金项目(31201929);山东省“双一流”奖补资金

摘　　要：以抗鼠伤寒沙门菌EF-Tu蛋白的单克隆抗体或多克隆抗体作为检测抗体,应用蛋白质印迹、间接免疫荧光试验和免疫溶菌试验等方法鉴定EF-Tu是否存在于菌体细胞表面。结果显示,鼠伤寒沙门菌EF-Tu蛋白可以定位到细菌表面;这是首次证实胞内蛋白EF-Tu可以分泌到鼠伤寒沙门菌菌体表面。这为进一步探究EF-Tu蛋白在沙门菌侵入及胞内存活过程中的调控作用及其分子机制奠定了基础。The monoclonal or polyclonal antibodies resistance to Salmonella typhimurium EF-Tu was used as a detection antibody,and the target protein EF-Tu on the bacterial surface was confirmed using Western-blot,indirect immunofluorescence assay,and complement-mediated immune lysis test.These results showed that the EF-Tu protein was located on the surface of S. typhimurium. This is believed to be the first report that intracellular protein EF-Tu could be secreted and located on the surface of S.typhimurium.This study laid the foundation for further investigation of the regulatory effect and molecular mechanism of EF-Tu protein in the invasion and intracellular survival of Salmonella.

关 键 词：鼠伤寒沙门菌 EF-TU 表面鉴定 

分 类 号：S852.612[农业科学—基础兽医学]