国产与泰国薏苡仁DNA条形码的比较  

Comparison of DNA barcodes of domestic and Thailand Semen Coicis

作　　者：朱晓燕[1] 陈伟盛[1] 黄韵璇 侯惠婵[1] ZHU Xiaoyan;CHEN Weisheng;HUANG Yunxuan;HOU Huichan(Guangzhou Institute for Drug Control,Guangzhou 510160,China)

机构地区：[1]广州市药品检验所,广州510160

出　　处：《中国药品标准》2018年第5期325-329,共5页Drug Standards of China

摘　　要：目的:通过对国产薏苡仁和泰国薏苡仁四段DNA条形码序列的比较,探索两者的亲缘关系,为其基因型上的差别提供佐证。方法:采用聚合酶链式反应(PCR)扩增两种薏苡仁的ITS2、psbA-trnH、rbcL、matK和ITS四段保守基因,并对其PCR产物进行测序和比对。结果:四段基因的PCR扩增和测序成功率均为100%,且国产薏苡仁与泰国薏苡仁的四段基因序列无位点差异。结论:国产薏苡仁与泰国薏苡仁的亲缘关系极近,或可一定程度上替代国产薏苡仁。Objective： To explore the vide the evidences of DNA differences genetic relationship between domestic and Thailand Semen Coicis and pro- through the sequence alignment of four DNA fragments barcodes. Methods： The polymerase chain reaction （PCR） was used to amplify DNA sequences of ITS2, psbA-trnH, rbcL, marK and ITS conserved gene of domestic Semen Coicis and Thailand Semen Coicis, and PCR product sequencing and align- ment were performed. Results： The success rates of PCR amplification and sequencing for ITS2, psbA-trnH, rbcL, and matK were 100%, and there was no difference on the DNA sequences sites between domestic Semen Coicis and Thailand Semen Coicis. Conclusion： There was very close genetic relationship between domestic Semen Coicis and Thailand Semen Coicis. It is probable that Thailand Semen Coicis could substitute domestic Semen Coicis in the market.

关 键 词：国产薏苡仁 泰国薏苡仁 聚合酶链式反应(PCR) DNA条形码 亲缘关系 序列比对 

分 类 号：R921.2[医药卫生—药学]