4种不同滤料对水泥粉尘致小鼠肺损伤防护效果  

Protective effect of four filter materials on lung injury induced by cement dust in mice

作　　者：李欣 李铁骥[2] 刘诗毓 张祥[2] LI Xin;LI Tie-ji;LIU Shi-yu;ZHANG Xiang(Jilin Safety Inspection and Testing Center Co.,Ltd.,Changchun Jilin,130022,China;Department of Labor and Environmental Hygiene,School of Public Health,Jilin University,Changchun Jilin,130021,China)

机构地区：[1]吉林省安全生产检测检验股份有限公司,长春吉林130022 [2]吉林大学公共卫生学院劳环卫教研室,长春吉林130021

出　　处：《职业与健康》2018年第20期2777-2781,共5页Occupation and Health

摘　　要：目的探讨4种不同滤料对水泥粉尘致小鼠肺损伤的防护效果,选择保护效果最好的防尘口罩滤料。方法将36只雄性ICR小鼠随机分为6组,分别为:对照组(不染毒)、染毒组(染毒28 d)、保护1组(医用纱布滤料,染毒28 d)、保护2组(无纺布滤料,染毒28 d)、保护3组(活性炭无纺布滤料,染毒28 d)、保护4组(植物油和凡士林浸润纱布滤料,染毒28 d)。末次染毒结束,禁水禁食24 h后称重、处死,测血清超氧化物歧化酶活性、肺组织羟脯氨酸含量及病理学改变。结果①染毒组、对照组、保护1组、保护2组、保护3组、保护4组SOD活力分别为(182.67±13.82、165.05±5.81、182.67±13.82、174.78±14.45、165.89±25.96、163.48±11.46、165.39±9.60)U/ml。经统计学分析,染毒组SOD活力高于对照组(P<0.05)。保护2组、保护3组、保护4组SOD活力均低于染毒组(P<0.05),保护1组与染毒组相比差异无统计学意义(P>0.05);②染毒组、对照组、保护1组、保护2组、保护3组、保护4组羟脯氨酸含量分别为(0.84±0.05、0.50±0.07、0.58±0.04、0.58±0.07、0.50±0.05、0.48±0.04)μg/ml。经统计学分析与对照组相比,保护1组羟脯氨酸含量升高,其他各组差异无统计学意义(P>0.05)。与染毒组相比,各保护组的羟脯氨酸含量均降低(P<0.05)。各保护组两两比较,保护3组和保护4组羟脯氨酸含量均低于保护1组(P<0.05),保护4组羟脯氨酸含量低于保护2组(P<0.05)。③病理学结果显示,对照组为正常肺组织结构、染毒组有疑似尘细胞出现,对照组为正常肺组织结构,保护1组和保护2组有数量略多巨噬细胞出现,保护3组和保护4组为正常肺组织结构。结论以植物油凡士林浸润的纱布和活性炭为原料的防尘口罩防护效果最好,其次为活性炭滤料,无纺布防护效果较差,纱布基本无防护效果。[Objective]To investigate the protective effect of four filter materials on lung injury induced by cement dust in mice,choose the dust respirator filter material with best protection effect.[Methods]36 male ICR mice were randomly divided into six groups： control group（no dust）,exposure group（no protected）,protected group 1（medical gauze）,protected group 2（non-woven fabrics）,protected group 3（active carbon）,protected group 4（gauze impregnated with vegetable oil and Vaseline）,the exposure group and protected groups were exposed for 28 days. At the end of the last exposure,the animals were weighed and killed after prohibition of water and fasting for 24 hours. The vitality of serum Superoxide Oxide Dismutase（SOD）,the concentrations of hydroxyproline in lung and the pathology changes of lung tissue were examined.[Results]（1）The SOD vitality in exposure group,control group,protected gro up 1,protected group 2,prot ected group 3,protected group 4 was（182.67 ±13.82,165.05 ±5.81,182.67 ±13.82,174.78 ±14.45,165.89 ±25.96,163.48 ±11.46,165.39 ±9.60） U/ml. The statistical analysis results showed that SOD vitality in exposure group was higher than that in control group（P〈0.05）. The SOD vitality in protected group 2,protected group 3,protected group 4 was lower than that in exposure group（P〈0.05）,and there was no statistically significant difference between protected group 1 and exposure group（P〈0.05）.（2）The concentrations of hydroxyproline in exposure group,control group,protected group 1,protected group 2,protected group 3,protected group 4 was（0.84±0.05,0.50±0.07,0.58±0.04,0.58±0.07,0.50±0.05 and 0.48 ±0.04） μg/ml,respectively. Compared with the control group,t he concentrations of hydroxyproline in protected group 1 increased,and there was no statistically significant difference between the control group and other groups（P〈0.05）.Compared with the exposure group,the concentrations of hydroxyproline in all protected groups decreased（P〈0.

关 键 词：水泥粉尘 滤料 超氧化物歧化酶 羟脯氨酸 

分 类 号：R114[医药卫生—卫生毒理学]