SLE患者外周血单个核细胞中miR-148b、miR-152和靶基因dnmt1的表达及其临床意义  

The expression and clinical significance of miR-148b, miR-152 and its target gene dnmt1 in peripheral blood mononuclear cells of patients with systemic lupus erythematosus

作　　者：杜明[1] 陶李[1] 王昱斌 李春莳 李光迪[1] DU Ming;TAO Li;WANG Yubing;LI Chunshi;LI Guangdi(Department of Clinical Laboratory,Lanzhou University Second Hospital,Lanzhou 730030,China)

机构地区：[1]兰州大学第二医院检验中心,730030

出　　处：《免疫学杂志》2018年第11期966-971,983,共7页Immunological Journal

摘　　要：目的探讨系统性红斑狼疮(systemic lupus erythematosus,SLE)患者外周血单个核细胞中miR-148b、miR-152和其靶基因dnmt1的表达水平在发病机制中的作用以及与SLE实验室相关指标、临床疾病活动度的之间的关系。方法采用实时荧光定量PCR(quantitative real-time,qPCR)方法检测74例SLE患者和44例健康者的PBMCs中miR-148b、miR-152、dnmt1的表达水平,统计学分析其与SLE疾病活动度及SLE相关检验指标的关系。结果 miR-148b、miR-152在SLE患者PBMCs中的表达水平显著高于健康对照(P<0.05),dnmt1在SLE患者PBMCs中的表达水平显著低于健康对照(P<0.05)。除miR-148b在低活动组与健康对照组差异无统计学意义(P>0.05)外,miR-148b、miR-152和dnmt1在其他各组中的表达差异均有统计学意义。miR-152与ESR正相关(r=0.443,P=0.027);dnmt1与ANA抗体(r=-0.576,P=0.001)及SLEDAI评分均为负相关(r=-0.408,P=0.038)。结论 miR-148b、miR-152和dnmt1在SLE的发病机制中扮演着重要角色,可能作为疾病活动的参考指标,有助于指导疾病治疗和预后评估,并为SLE的治疗带来更多靶点。This study designed to investigate the roles of miR-148 b, miR-152 and its target gene dnmt1 in the pathogenesis of systemic lupus erythematosus（SLE）, and their relationship with SLE laboratory indicators and disease-activity indexes. Total of 74 cases of SLE and 44 healthy controls were recruited in the study. Quantitative real-time（qPCR） was used to detect the expression levels of miR-148 b, miR-152 and dnmt1; the correlation of miR-148 b, miR-152 or dnmt1 with every laboratory indicator was analyzed. Data showed that the expression levels of miR-148 b and miR-152 in PBMCs of SLE patients were significantly higher than those in healthy controls（P〈0.05）, while the expression of dnmt1 in PBMCs of SLE patients was significantly lower than that in healthy controls（P〈0.05）. Except that miR-148 b demonstrated no significant difference between low activity group and healthy control group（P〈0.05）, the expression of miR-148 b, miR-152, and dnmt1 were significant different in these groups.Statistic analysis showed that miR-152 positively correlated with ESR（r=0.443, P=0.027）, while dnmt1 was negatively correlated with ANA antibody（r=-0.576, P=0.001） and SLEDAI（r=-0.408, P=0.038）. The present study demonstrates that miR-148 b, miR-152 and dnmt1 may play critical roles in the pathogenesis of SLE, and can be used as a reference index of disease activity, thus contributing to the treatment and prognosis guide of disease, and lead to more therapeutics for this disease.

关 键 词：系统性红斑狼疮 miR-148b miR-152 DNMT1 

分 类 号：R593.241[医药卫生—内科学]