银屑病患者皮损间充质干细胞磷脂酶C-β4和视黄醇脱氢酶10表达水平的研究  

Expression of PLCB4 and RDH10 in dermal mesenchymal stem cells of psoriatic patients

作　　者：李娇[1] 李娟[1] 梁见楠 杨晓红[1] 侯瑞霞[1] 王颖 焦娟娟 张开明[1] LI Jiao;LI Juan;LIANG Jian-nan;YANG Xiao-hong;HOU Rui-xia;WANG Ying;JIAO Juan-juan;ZHANG Kai-ming(Department of Dermatology,Shanxi Key Laboratory of Stem Cell for Immunological Dermatosis,Taiyuan City Central Hospital,Taiyuan 030009,China)

机构地区：[1]太原市中心医院皮肤科免疫性皮肤病干细胞山西省重点实验室,山西太原030009

出　　处：《临床皮肤科杂志》2018年第11期700-704,共5页Journal of Clinical Dermatology

基　　金：国家自然科学基金(81602768)资助项目

摘　　要：目的:通过研究银屑病患者皮损处真皮间充质干细胞(DMSCs)中磷脂酶C-β4(PLCB4)和视黄醇脱氢酶(RDH)10mRNA的表达水平,进一步探讨银屑病发病的细胞及分子机制。方法:对24例寻常性银屑病(PV)患者和22例正常人皮肤DMSCs进行分离培养,流式细胞术进行细胞表型鉴定,实时荧光定量聚合酶链式反应(PCR)测定PLCB4和RDH10 mRNA表达水平。结果:PV组与正常对照组DMSCs形态无差异。PV组患者DMSCs中PLCB4 m RNA的表达水平是正常对照组的3.35倍,RDH10 mRNA的表达水平是正常对照组的1.17倍。结论:银屑病患者DMSCs中PLCB4和RDH10 m RNA表达均增高,可能影响相关信号通路,进而对银屑病发病产生影响。Objective： To assess expression levels of phospholipase C-beta 4（PLCB4） and retinol dehydrogenase 10（RDH10） mRNA in dermal mesenchymal stem cells（DMSCs） from lesional skin of psoriatic patients in order to elucidate the cellular and molecular pathomechanisms of psoriasis. Methods： DMSCs from 24 patients with psoriasis vulgaris and 22 normal controls were cultured. Cell phenotypes were identified by flow cytometry, while expression levels of PLCB4 and RDH10 mRNA were determined using real-time fluorescence quantitative PCR. Results： While the morphology of DMSCs was similar between the two groups, expression levels of PLCB4 mRNA in psoriatic DMSCs were 3.35 folds of that in normal controls. Expression levels of RDH10 mRNA in psoriatic DMSCs were 1.17 folds of that in normal controls. Conclusion： The increased expression levels of PI,CB4 and RDH10 in psoriatic DMSCs suggest that these two molecules may involve in the pathogenesis of psoriasis via regulating respective signaling pathway.

关 键 词：银屑病 间充质干细胞 磷脂酶C-β4 视黄醇脱氢酶10 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]