油松FT/TFL1-like基因表达模式与调控分析  

Expression profiles and regulation of FT/TFL1-like genes in Pinus tabuliformis

作　　者：刘洋 李帮同 杜桂华[3] 黄东旭 周先清 钮世辉[1] 李伟[1] Liu Yang;Li Bangtong;Du Guihua;Huang Dongxu;Zhou Xianqing;Niu Shihui;Li Wei(National Engineering Laboratory for Forest Tree Breeding,Beijing Forestry University,Bcijing 100083,China;Forestry Seedling Management Station,Taiyuan 030001,Shanxi,China;Agriculture and Animal Husbandry Bureau,Ningcheng 024200,Inner Mongolia,China;National Fine Seed Base of Pinus tabuliformis,Qigou State-owned Forest Farm,Pingquan 067509,Hebei,China)

机构地区：[1]北京林业大学林木育种国家工程实验室,北京100083 [2]山西省林业种苗管理总站,山西太原030001 [3]内蒙古宁城县农牧业局,内蒙古宁城024200 [4]河北省平泉市国有七沟林场国家油松良种基地,河北平泉067509

出　　处：《北京林业大学学报》2018年第10期60-66,共7页Journal of Beijing Forestry University

基　　金：中央高校基本科研业务费专项资金项目(2015ZCQ-SW-02);国家自然科学基金项目(31600535;31770713)

摘　　要：【目的】PEBP基因家族的FT-like与TFL1-like亚基因家族在被子植物生殖发育调控网络中处于核心调控节点,而在针叶树中,仅存在未分化的FT/TFL1-like亚基因家族。研究表明针叶树FT/TFL1-like可能在生殖发育、生长节律及休眠过程中发挥重要功能。然而,目前关于针叶树FT/TFL1-like基因系统的表达模式与不同环境因子对其表达的调控仍然缺乏深入认识。【方法】本研究从油松中分离得到两个FT/TFL1-like基因,分别命名为Pt TFL1与Pt TFL2,对它们在不同组织、不同发育阶段雌雄球花、休眠与解除休眠过程中针叶、深冬相对高温、不同光质与光周期处理中的表达水平进行了系统分析。【结果】Pt TFL1仅在花粉中特异表达,在其他组织中只有痕量表达或不表达。而Pt TFL2表达范围非常广泛,且在芽组织中的表达丰度显著高于其他组织,二者在快速增殖的愈伤组织中均无明显表达;另外,对Pt TFL2在不同环境下表达水平进行分析发现,Pt TFL2在芽、针叶休眠时期大量积累,且随着休眠解除表达量水平持续降低;在不足以打破休眠的相对高温处理下,Pt TFL2的表达受到极显著的抑制;短日照下远红光处理可以高效诱导Pt TFL2的表达。【结论】由此可知,Pt TFL1在油松生殖发育进程中的特异性可能与花粉成熟过程相关,而Pt TFL2可能参与更多其他发育调控,但二者均不是维持细胞活性所必须的基因;秋季环境条件非常利于Pt TFL2在针叶、芽中大量表达,其表达模式与休眠过程高度相关,但Pt TFL2对温度响应过于敏感,其在针叶、雌雄球花中的表达模式很可能是对温度响应的结果,这表明Pt TFL2自身的表达可能并不足以维持休眠,也并不作为球花发育调控中的控制因子,而很可能只是作为一个温度、短日照远红光信号的感受与传递因子。本研究为深入理解FT/TFL1-like基因在油松生殖与休眠等重要发育过程中的功�[Objective]There are three subfamilies of the PEBP gene family： FT-like,TFL1-like and FT/TFL11-like. We have known that FT/TFL1-like genes are the core regulation hubs in reproductive regulation network in angiosperms. Only the undifferentiated FT/TFL1-like subfamily in conifers wassuggested play important roles of reproduction,growth rhythm and dormancy process. However,the knowledge of its expression profiles and the expressional regulation by different environmental factors about the FT/TFL1-like genes in conifers are still limited. This may result in some inaccurate speculation about the role of its related expression mechanism. [Method]In this study,we isolated two FT/TFL1-like genes form Chinese pine（ Pinus tabuliformis）,named as Pt TFL1 and Pt TFL2. The expression level of these genes in different tissues,the developing male cones and female cones in different stage,the needles during the dormancy and dormancy release,the response to relative warmer temperature and under different day length and light were studied. [Result]The results showed that Pt TFL1 was specifically expressed in pollen and nearly no expression in other tissues. But the Pt TFL2 was very broadly expressed and significantly expressed in the bud tissue than other tissues. But both of two genes were not significantly expressed in the rapidly proliferating callus; in addition,the analysis of the expression level of Pt TFL2 in different environments was found. Pt TFL2 accumulated in buds and conifers during the dormancy period and decreased when dormancy release; under the condition of relatively high temperature but not enough to break dormancy,the expression of Pt TFL2 was significantly inhibited,which was similar to that of far red light treatment. [Conclusion]It can be seen that Pt TFL1 may be related to pollen maturation process in the reproductive development process of Pinus tabuliformis,but Pt TFL2 may be involved in more other developmental regulation,both of them are not necessary to maintain cell activity. The autumn environ

关 键 词：FT/TFL1-like基因 PtTFL2 表达模式 表达调控 油松 

分 类 号：S722[农业科学—林木遗传育种]