羊草DHN3基因的克隆及其逆境响应的表达分析  

Cloning of LcDHN3 in Leymus chinensis and Its Expression under Abiotic Stresses

作　　者：万永青 张杰[1] 侯向阳 王照兰[1] 马玉宝[1] 万其号[1] 万东莉[1] WAN Yongqing;ZHANG Jie;HOU Xiangyang;WANG Zhaolan;MA Yubao;WAN Qihao;WAN Dongli(Institute of Grassland Research,Chinese Academy of Agricultural Sciences,Key Laboratory of Grassland Ecology and Restoration,Ministry of Agriculture,Hohhot 010010,China;College of Life Sciences,Inner Mongolia Agricultural University,Inner Mongolia Key Laboratory of Plant Stress Physiology and Molecular Biology,Hohhot 010011,China)

机构地区：[1]中国农业科学院草原研究所农业部草地生态与修复治理重点实验室,呼和浩特010010 [2]内蒙古农业大学生命科学学院内蒙古自治区植物逆境生理与分子生物学重点实验室,呼和浩特010011

出　　处：《西北植物学报》2018年第9期1598-1604,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：中国农业科学院科技创新工程(CAAS-ASTIP-IGR);中央级公益性科研院所基本科研业务费项目(1610332016018)

摘　　要：该研究从羊草(Leymus chinensis)中克隆得到1个脱水素(Dehydrin,DHN)编码基因LcDHN3。通过序列分析表明,LcDHN3开放阅读框为501bp,编码167个氨基酸,分子量为17.01kD,理论等电点为8.05,为亲水性蛋白。LcDHN3蛋白具有脱水素的保守结构域,含有1个Y-片段、1个S-片段和2个K-片段,属于YSK2型脱水素。亚细胞定位预测LcDHN3蛋白定位在细胞质和细胞核。同源比对分析显示,LcDHN3与大麦(Hordeum vulgare)等6种植物的DHNs整体相似性为84.27%。进化分析显示,LcDHN3和大麦的DHN亲缘关系最近。荧光定量PCR结果显示,LcDHN3基因的表达受干旱、冷、热、NaCl、高pH和机械损伤胁迫诱导,同时受植物激素ABA和JA的诱导。研究表明,LcDHN3参与了羊草响应逆境胁迫的信号途径。In this study,we cloned a dehydrin encoding gene LcDHN3 fromLeymus chinensis.The sequence analysis shows that the open reading frame of LcDHN3 is 501 bp and encodes 167 amino acids,molecular weight is 17.01 kD,theoretical pI is 8.05,contains dehydrin superfamily domain,and one Y-segment,one S-segment and two K-segments,belongs to YSK2 type dehydrin.Subcellular localization predicts LcDHN3 may localized in both cytoplasm and nuclei.Multiple alignment of homology sequences showed that the identity of LcDHN3 with DHNs from other plants was 84.27%.Phylogenetic analysis revealed LcDHN3 had the closest genetic relationship with Hordeum vulgare DHN.The transcripts of LcDHN3 were induced by different abiotic stresses,including drought,cold,heat,NaCl,high pH and wounding,as well as ABA and JA treatments.These results indicate that LcDHN3 involved in the signal transduction pathway of L.chinensis in response to abiotic stresses.

关 键 词：脱水素 基因克隆 逆境胁迫 表达分析 羊草 

分 类 号：Q785[生物学—分子生物学] Q786