矮牵牛PhTPS5基因的克隆与表达分析  

Identification and Expression Analysis of PhTPS5 in Petunia

作　　者：刘娜 王琦 刘同瑞 熊枫 张水明[1] 董丽丽[1] LIU Na;WANG Qi;LIU Tongrui;XIONG Feng;ZHANG Shuiming;DONG Lili(College of IIorticulture,Anhui Agricultural University,Hefei 23003G,China)

机构地区：[1]安徽农业大学园艺学院,合肥230036

出　　处：《西北植物学报》2018年第9期1620-1624,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：安徽省高等学校自然科学研究重点项目(KJ2017A154)资助

摘　　要：海藻糖-6-磷酸合成酶(Trehalose-6-phosphate synthase)基因TPS是海藻糖生物合成途径中的关键基因之一。该研究从矮牵牛(Petunia hybrida)中分离了TPS5的同源基因PhTPS5。该基因开放阅读框(ORF)为2 595bp,编码864个氨基酸。推测PhTPS5蛋白的分子式为C4363H6825N1173O1289S37。组织特异性表达分析显示:PhTPS5基因在根中表达量最高,叶片中的表达量最低;去顶6h能够显著促进PhTPS5基因的表达,但24h后表达量明显下降;去顶后施加生长素则能够有效抑制去顶对PhTPS5基因表达的调节;施加细胞分裂素6h后PhTPS5基因的表达水平显著上调,但随着处理时间的增加,其表达水平有所下降。该研究为进一步揭示TPS途径在矮牵牛分枝发育中的调控机制奠定了理论基础。Trehalose-6-phosphate synthase gene TPSis one of the key genes for the synthesis of trehalose.In this study,the homologue of TPS5 was isolated from Petunia hybrida,which was named PhTPS5.The open reading frame（ORF）of PhTPS5 was 2595 bp and encoded a protein of 864 amino acids.The molecular formula of PhTPS5 protein was assumed to be C4363 H6825 N1173 O1289 S37.Tissue-specific expression analysis showed that PhTPS5 had the highest expression level in roots and the lowest in leaves.After 6 h of decapitation,PhTPS5 expression was significantly promoted,and the expression level decreased significantly at 24 h.The application of auxin to the top could effectively inhibit the regulation to PhTPS5.The expression level of PhTPS5 was significantly up-regulated after 6 hof cytokinin treatment,and decreased with the increase of treatment time.This study laid a theoretical foundation for further revealing the regulatory mechanism of the TPS pathway in the shoot branching of petunia.

关 键 词：矮牵牛 分枝发育 PhTPS5 生长素 细胞分裂素 表达分析 

分 类 号：Q785[生物学—分子生物学] Q786